人脐带间充质干细胞对舌鳞状细胞癌Cal-27细胞侵袭和迁移能力的影响  

Effect of Human Umbilical Cord Mesenchymal Stem Cells on Invasion and Migration of Cal-27 Cells from Tongue Squamous Cell Carcinoma

作　　者：张馨予[1] 吴双燕 吴鸿[1] 徐竹青 王云英[3] 徐晓娜[3] 李新[1] 郑建金[1] ZHANG Xin-yu;WU Shuang-yan;WU Hong;XU Zhu-qing;WANG Yun-ying;XU Xiao-na;LI Xin;ZHENG Jian-jin(School of Stomatology of Qingdao University,Qingdao 266003,China;Qingdao Stomatological Hospital,Qingdao 266001,China;Central Laboratory of Qingdao Municipal Hospital,Qingdao 266000,China.)

机构地区：[1]青岛大学口腔医学院,山东青岛266003 [2]青岛市口腔医院多学科,山东青岛266001 [3]青岛市市立医院中心实验室,山东青岛266000

出　　处：《口腔医学研究》2019年第5期443-447,共5页Journal of Oral Science Research

基　　金：青岛市科技局重点项目(编号:15-9-2-79-nsh)

摘　　要：目的:探讨人脐带间充质干细胞(human umbilical cord blood mesenchymal stem cells,HUC-MSCs)对舌鳞状细胞癌Cal-27细胞侵袭和迁移能力的影响。方法:细胞划痕实验检测HUC-MSCs分泌的细胞因子对Cal-27细胞迁移能力的影响;Transwell小室比较共培养前后Cal-27细胞的侵袭和迁移能力;逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)检测共培养前后Cal-27细胞的侵袭、迁移相关基因的表达水平;Western blot技术检测肿瘤侵袭转移相关蛋白分子基质金属蛋白酶(matrix metalloproteinase,MMP)-2和MMP-9的蛋白表达的情况。结果:条件培养基培养的Cal-27细胞的迁移距离短于对照组;共培养后Cal-27细胞的侵袭和迁移细胞数目减少;侵袭迁移相关基因、蛋白的表达水平下调。结论:HUC-MSCs与Cal-27细胞共培养可以抑制Cal-27细胞的侵袭和迁移。Objective:To investigate the effect of human umbilical cord mesenchymal stem cells(HUC-MSCs)on the invasion and migration of Cal-27 cells in tongue squamous cell carcinoma.Methods:HUC-MSCs conditioned medium was prepared.Cal-27 cells were cultured and the effect of HUC-MSCs secreted factors on Cal-27 cell migration was detected by cell scratch test.The invasiveness and migration of Cal-27 cells before and after co-culture were compared in Transwell cells.Expression levels of genes related to invasion and migration of Cal-27 cells were detected by RT-PCR before and after co-culture.The changes of MMP2 and MMP9 protein expression in tumor invasion and metastasis-related protein molecules were detected by western blot.Results:The migration distance of Cal-27 cells was shorter than that of the control group.After co-culture,the number of invasion and migration of Cal-27 cells decreased.The expression levels of aggression-related genes and proteins were down-regulated.Conclusion:The co-culture of HUC-MSCs and Cal-27 cells can inhibit the invasion and migration of Cal-27 cells.

关 键 词：人脐带间充质干细胞 舌鳞癌细胞Cal-27 条件培养基 侵袭和迁移 基质金属蛋白酶 

分 类 号：R739.86[医药卫生—肿瘤]