诱导大鼠脂肪来源干细胞向腺泡样细胞转化的实验研究  

Study on Transdifferentiation of Adipose-derived Stem Cells into Salivary Gland Acinar-like Cells

作　　者：于阳[1] 徐文品 焦琨[1] 刘冬蕊[1] 邢如霄 叶佳朋 张伟[1] YU Yang;XU Wen-pin;JIAO Kun;LIU Dong-rui;XING Ru-xiao;YE Jia-peng;ZHANG Wei(Department of Oral and Maxillofacial Surgery,Stomatological Hospital,Jilin University,Changchun 130021,China.)

机构地区：[1]吉林大学口腔医院颌面外科,吉林长春130021

出　　处：《口腔医学研究》2019年第5期493-496,共4页Journal of Oral Science Research

基　　金：省厅级项目基金(编号:3D5139073431)

摘　　要：目的:研究脂肪来源干细胞(adipose-derived stem cells,ADSCs)在下颌下腺细胞submandubular gland cells,SMGCs)裂解液模拟的微环境下能否转化为具有分泌功能的腺泡样细胞。方法:提取,培养大鼠的ADSCs并鉴定,用下颌下腺细胞裂解液诱导其分化,观察其形态变化以及是否表达SMGCs的α-Amylase抗体及上皮细胞来源的Cytokeratin-8抗体,并用淀粉酶检测试剂盒检测诱导不同时间细胞上清液内淀粉酶的含量,分析转化效率。结果:自大鼠脂肪组织提取的细胞具有多向分化的能力,证实其为脂肪来源干细胞,经下颌下腺细胞裂解液诱导后,α-Amylase抗体及Cytokeratin-8抗体表达阳性,而对照组为阴性,证实其成功转化为腺泡样细胞,随着时间增加细胞上清液内淀粉酶含量逐渐增加,说明其诱导效率随着时间增长而逐渐增高。结论:脂肪来源干细胞可以转化为具有分泌,α-Amylase功能的腺泡样细胞,随着时间增长其转化效率逐渐提高。Objective:To investigate whether adipose-derived stem cells(ADSCs)can be transformed into acinar-like cells with secretory function in the microenvironment simulated by submandubular gland cells(SMGCs).Methed:ADSCs were isolated from neonatal SD rats and cultured in vitro.Submandibular gland cell lysate was used to induce differentiation.To observe the morphological changes and expression ofα-amylase antibody and cytokeratin-8 antibody,the content of amylase in the supernatant of cells induced at different periods was detected using amylase detection kit,and the conversion efficiency was analyzed.Results:The cells extracted from adipose tissue of rats had the ability to differentiate into multiple directions,which confirmed that they were adipose derived stem cells.After induced by submandibular gland cell lysate,α-amylase antibody and cytokeratin-8 antibody staining were positive,but negative in control group.This confirmed that the cells were successfully transformed into acinar-like cells,and the content of amylase in the supernatant increased with time,which indicated that the induction efficiency increased with time.Conclusion:Adipose derived stem cells can be transformed into acinar-like cells with secretory andα-amylase function,and their transformation efficiency increased with time.

关 键 词：脂肪来源干细胞 下颌下腺细胞裂解液 细胞分化 

分 类 号：R78[医药卫生—口腔医学]