许氏平鲉发育早期的氨基酸与脂肪酸组成及变化  

ANALYSIS ON THE COMPOSITION OF AMINO ACID AND FATTY ACID AND THEIR CHANGES DURING EARLY GROWTH STAGE OF SEBASTES SCHLEGELII

作　　者：韩慧宗 王腾腾 张明亮 王斐 刘阳 孙娜[1,2] 姜海滨 HAN Hui-Zong;WANG Teng-Teng;ZHANG Ming-Liang;WANG Fei;LIU Yang;SUN Na;JIANG Hai-Bin(The Key Laboratory of Restoration for Marine Ecology of Shandong Province, Shandong Marine Resource and Environment Research Institute, Yantai 264006, China;College of Fisheries and Life Science, Shanghai Ocean University,Shanghai 201306, China)

机构地区：[1]山东省海洋资源与环境研究院山东省海洋生态修复重点实验室,烟台264006 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《水生生物学报》2019年第3期526-536,共11页Acta Hydrobiologica Sinica

基　　金：山东省自然科学基金(ZR2016CP21);现代农业产业技术体系专项(CARS-47-Z14);山东省重点研发计划项目(2017GHY15107);山东省农业重大应用技术创新项目"高山岛复合型生态海洋牧场构建与示范"(2017-2020)资助~~

摘　　要：为了研究许氏平鲉(Sebastes schlegelii)雌鱼体内受精后仔鱼开口前和仔鱼开口后两个阶段氨基酸和脂肪酸的组成变化规律,采用生化常规方法定量测定并分析了许氏平鲉发育早期的受精卵(FE)、胚胎期(ES)、初产仔鱼(PL_1)、前仔鱼期(PL_2)、后仔鱼期(PL_3)和稚鱼期(J)6个阶段的氨基酸和脂肪酸组成特点及含量变动。结果表明:总氨基酸含量从FE至PL_1显著下降,至PL_3显著上升,至J又显著下降(P<0.05);游离氨基酸含量以FE最低(12.77 mg/g),从FE到PL_1显著上升(P<0.05),并在PL_1含量达到最高值(92.19 mg/g), PL_1发育到J呈现先下降后上升再下降的变化趋势(P<0.05),游离氨基酸与总氨基酸的比值范围在2.37%—19.66%。在各发育阶段干样中检出碳链长度在C_(14)-C_(24)的29种脂肪酸,分别为9种饱和脂肪酸(SFA)、9种单不饱和脂肪酸(MUFA)和11种多不饱和脂肪酸为(PUFA),受精卵中主要脂肪酸依次为C_(22:6n-3)(DHA)、C_(18:ln-9c)、C_(16:0)和C_(20:5n-3)(EPA)。胚胎期(FE-ES)的脂肪酸利用率顺序为SFA、MUFA、n-6PUFA、n-3PUFA,主要以C_(18:3n-3)、C_(18:0)、C_(16:1n-7)及C_(20:5n-3)(EPA)作为胚胎期的能量来源, C_(22:6n-3)(DHA)的实际利用率最低(9.71%),被优先保存下来,C16:0的实际利用量最高(10.94mg/g);仔鱼内源营养阶段(ES-PL_1)脂肪酸利用率顺序为MUFA、n-6PUFA、SFA、n-3PUFA,主要以C_(16:1n-7)、C_(18:0)、C_(20:4n-6)(ARA)及C_(18:1n-9c)作为开口前仔鱼的主要能量来源,其中仔鱼对DHA实际利用量最高(18.23 mg/g)。PL_1-PL_3阶段DHA相对于EPA和ARA被选择性利用; PL_3至J阶段ARA相对于EPA和DHA被选择性利用。研究表明:许氏平鲉仔鱼开口前阶段总氨基酸含量与游离氨基酸含量的变化趋势截然相反,胚胎期与仔鱼内源营养阶段脂肪酸利用率和利用量均有所不同,仔鱼期DHA优先被利用,过渡至稚鱼期ARA优先被利用。建议在仔鱼开口后添加富含DHA生物性饵料,仔鱼过渡到稚鱼期在配�The black rockfish (Sebastes schlegelii) has been an important economic marine fish species of deepwater cage farming, enhancement releasing and fishing in the northern coast of China. Its reproduction is ovoviviparous, dur- ing which the embryo is produced in female fish and usually grow to free fish around May. There is no report on the nutritional requirements and metabolic characteristics of the early development of S. schlegelii. In order to investigate the compositions of amino acids and fatty acids as well as their changes for S. schlegelii in different developmental stages, related analysis at different stages including fertilized eggs (FE), embryo stage (ES), primiparous larvae (PL1), pre-larva (PL2), post-larva (PL3) and juvenile (J) were conducted by biochemical analysis methods. The S. schlegelii was sampled at the breeding demonstration base of Shandong Marine Resource and Environment Research Institute, Yantai, Shandong Province from April to July in 2016. The results showed that the total amino acid content decreased significantly from FE to PL1, and then increased significantly during prophase PL3, and finally had another significant decrease in the late stage of J (P<0.05). The total amount of free amino acid during FE was the lowest among the six stages (12.77 mg/g), which then significantly increased from FE to PL1 (P<0.05), and reached to the highest level of 92.19 mg/g, and after that it diminished first, then rose, and decreased again (P<0.05). The content of free essential amino acids was significantly higher than that of free nonessential amino acids at each developmental stage. The per- centage of free amino acids at each stage was 2.37% 19.66%. Twenty-nine fatty acids were detected in S. schlegelii, including 9 saturated fatty acids (SFA), 10 single unsaturated fatty acids (MUFA) and 11 poly unsaturated fatty acids (PUFA). The major fatty acids in fertilized eggs were C22:6n-3 (DHA), C18:ln-9c, C16:0 and C20:5n-3. At the embryonic pe- riod, the order of utilization rate of fatty acid was

关 键 词：许氏平鲉 氨基酸 脂肪酸 受精卵 胚胎期 仔鱼期 稚鱼期 利用率 

分 类 号：S965.3[农业科学—水产养殖]