Preparation and characterization of agar, agarose,and agaropectin from the red alga Ahnfeltia plicata  

作　　者：ZHANG Yu FU Xiaoting DUAN Delin XU Jiachao GAO Xin 

机构地区：[1]College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China [2]Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China [3]State Key Lab of Seaweed Bioactive Substances,Qingdao 266400,China

出　　处：《Journal of Oceanology and Limnology》2019年第3期815-824,共10页海洋湖沼学报（英文）

基　　金：Supported by the Public Science and Technology Research Funds Projects of Ocean(No.201405040);the Key Research and Development Program in Shandong Province(No.2016GSF121034);the Jiangsu Provincial Key R&D Project(No.BE2015335)

摘　　要：Agar,agarose,and agaropectin were extracted from the red alga Ahnfeltia plicata,and their properties and structures were characterized.Agar was extracted by a comparatively low alkaline consumption of 1.2%.It exhibited a gel strength of 1 152.50±74.25 g/cm^2 and a sulfate content of 0.55%±0.08%.The yield of agar from A.plicata was 24.53%,which is higher than those of other agarophytes commonly used in China.Three kinds of the method were compared for the purification of agarose,and the physicochemical properties of agarose that was prepared under the optimal condition were identical to those of commercially available agarose.Furthermore,agaropectin was purified from A.plicata and characterized by GC,HPLC,UV-spectrum,and FI-IR to understand its composition and structure.It was the first time to comprehensively study the agar and its fractions from the red alga of A.plicata.This research provided an eco-friendly agar extraction method from A.plicata and revealed its potential application for the production of agar,agarose,and agaropectin.Agar, agarose, and agaropectin were extracted from the red alga Ahnfeltia plicata, and their properties and structures were characterized. Agar was extracted by a comparatively low alkaline consumption of 1.2%. It exhibited a gel strength of 1 152.50±74.25 g/cm^2 and a sulfate content of 0.55%±0.08%. The yield of agar from A. plicata was 24.53%, which is higher than those of other agarophytes commonly used in China. Three kinds of the method were compared for the purification of agarose, and the physicochemical properties of agarose that was prepared under the optimal condition were identical to those of commercially available agarose. Furthermore, agaropectin was purified from A. plicata and characterized by GC, HPLC,UV-spectrum, and FI-IR to understand its composition and structure. It was the first time to comprehensively study the agar and its fractions from the red alga of A. plicata. This research provided an eco-friendly agar extraction method from A. plicata and revealed its potential application for the production of agar, agarose,and agaropectin.

关 键 词：Ahnfeltia plicata AGAR AGAROSE agaropectin 

分 类 号：P[天文地球]