Antioxidant and antitumor potential of wild and IMT-Acultivated Osmundea pinnatifida  

作　　者：SILVA J.Paulo ALVES Celso PINTEUS Susete SILVA Joana VALADO Ana PEDROSA Rui PEREIRA Leonel 

机构地区：[1]UCIBIO-REQUIMTE,Department of Chemistry,Faculty of Sciences and Technology,Universidade NOVA de Lisboa,Caparica 2829-516,Portugal [2]MARE-Marine and Environmental Sciences Centre,School of Tourism and Maritime Technology,Polytechnic Institute of Leiria,Peniche 2520-641,Portugal [3]Instituto Politécnico de Coimbra,ESTESC-Coimbra Health School,Biomedical Sciences Laboratory,R.5 de Outubro,S.Martinho do Bispo,Ap.7006,Coimbra 3046-854,Portugal [4]Centre of Pharmacology and Chemical Biopathology,Faculty of Medicine,University of Porto,Porto 4200-319,Portugal [5]MARE-Marine and Environmental Sciences Centre,Department of Life Sciences,Faculty of Sciences and Technology,University of Coimbra,Coimbra 3000-456,Portugal

出　　处：《Journal of Oceanology and Limnology》2019年第3期825-835,共11页海洋湖沼学报（英文）

基　　金：the support of Fundacao para a Ciência e Tecnologia(FCT),through the strategic project(No.UID/MAR/04292/2013)granted to MARE;the support from the European Union through EASME Blue Labs project AMALIA-Algae-to-MArket Lab IdeAs(No.EASME/EMFF/2016/1.2.1.4/03/SI2.750419);funding from European Structural&Investment Funds through the COMPETE Programme;National Funds through FCT-Fundagao para a Ciência e a Tecnologia under the Programme(No.SAICTPAC/0019/2015)

摘　　要：Osmundea pinnatifida is a red edible seaweed known as pepper dulse.O.pinnatifida was cultivated in the farm of ALGAplus(ílhavo,Portugal).This farm is integrated with a seabream and seabass commercial aquaculture and uses the nutrient-enriched water resultant from the fish production as its cultivation medium in the integrated multi-trophic aquaculture(IMTA)manner.Wild and IMTA-cultivated samples of O.pinnatifi da were screened for antioxidant and antitumor activities.The antioxidant capacity of solvent extracts from wild and IMTA cultivated samples was assessed in two methods(2,2-diphenyl-1-picrylhydrazyl(DPPH)and oxygen radical absorbance capacity(ORAC)),and their total phenolic contents(TPC)were estimated.Antitumor activity was evaluated in three different tumor cell lines(HepG-2,MCF-7,and SH-SY5Y)through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.Among the solvents used for extraction,dichloromethane was the most eff ective to extract phenolic compounds and presented higher ORAC.A significant correlation was found between TPC and ORAC,which was also sustained by the principal components analysis(PCA).Dichloromethane extracts induced a cytostatic eff ect on MCF-7 cells and showed weak cytotoxicity to SH-SY5Y cells and weak impact on cell proliferation.Overall,there were no statistically signifi cant differences in the biological activities shown by the wild and IMTA-cultivated samples.Hence,O.pinnatifida can be obtained in an economical and environmentally sustainable way through IMTA,maintaining bioactive properties in a high potential for further nutraceutical purposes.Osmundea pinnatifida is a red edible seaweed known as pepper dulse. O. pinnatifida was cultivated in the farm of ALGAplus(Ilhavo, Portugal). This farm is integrated with a seabream and seabass commercial aquaculture and uses the nutrient-enriched water resultant from the fish production as its cultivation medium in the integrated multi-trophic aquaculture(IMTA) manner. Wild and IMTA-cultivated samples of O. pinnatifida were screened for antioxidant and antitumor activities. The antioxidant capacity of solvent extracts from wild and IMTA cultivated samples was assessed in two methods(2,2-diphenyl-1-picrylhydrazyl(DPPH) and oxygen radical absorbance capacity(ORAC)), and their total phenolic contents(TPC) were estimated. Antitumor activity was evaluated in three different tumor cell lines(HepG-2, MCF-7, and SH-SY5Y) through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay. Among the solvents used for extraction, dichloromethane was the most effective to extract phenolic compounds and presented higher ORAC. A significant correlation was found between TPC and ORAC,which was also sustained by the principal components analysis(PCA). Dichloromethane extracts induced a cytostatic effect on MCF-7 cells and showed weak cytotoxicity to SH-SY5Y cells and weak impact on cell proliferation. Overall, there were no statistically significant differences in the biological activities shown by the wild and IMTA-cultivated samples. Hence, O. pinnatifida can be obtained in an economical and environmentally sustainable way through IMTA, maintaining bioactive properties in a high potential for further nutraceutical purposes.

关 键 词：edible seaweed bioactive compounds POLYPHENOLS cytotoxic activity antiproliferative activity 

分 类 号：P[天文地球]