抗坏血酸辅助碳纤维超微电极特异性检测特布他林  

Carbon fiber electrode for specific detection of terbutaline with the aid of ascorbic acid

作　　者：王婷婷[1] 李元[1] 王夏彤 程寒[1,2] WANG Tingting;LI Yuan;WANG Xiatong;CHENG Han(South-Central University for Nationalities,Wuhan 430074,Hubei,China;National Demonstration Center for Experimental Ethnopharmacology Education,Wuhan 430074,Hubei,China)

机构地区：[1]中南民族大学药学院,湖北武汉430074 [2]中南民族大学民族药学国家级实验教学示范中心,湖北武汉430074

出　　处：《化学研究》2019年第1期66-71,共6页Chemical Research

基　　金：中南民族大学学术团队(XTZ15013);中南民族大学硕士研究生创新基金项目(2018sycxjj214);中央高校基本科研业务费专项资金项目(CZY18036);民族医药学会项目(2017KYXM-M106-81;2017KYXM-Z182-82)

摘　　要：采用循环伏安法(CV)、差分脉冲伏安法(DPV)研究了特布他林与抗坏血酸共存的条件下在碳纤维超微电极上的电化学行为.通过优化抗坏血酸浓度,建立了一种电化学定量检测特布他林的方法.实验结果表明,在20 mmol/L pH 7.0的Tris-HCl缓冲溶液中,当共存的抗坏血酸浓度为1×10^(-4) mol/L时,对碳纤维电极检测特布他林具有较好的稳定作用.在优化条件下,采用DPV法对特布他林进行定量分析,特布他林的氧化峰电流与其浓度在1×10^(-6)～1×10^(-4) mol/L范围内呈良好的线性关系,线性相关系数为R^2=0.994 2,检测限达2.202×10^(-10) mol/L (S/N=3).该修饰方法重现性好,电极稳定性佳,可应用于生物样品中特布他林的高灵敏分析.The electrochemical behaviors of terbutaline and ascorbic acid coexist solution on a carbon fiber electrode was investigated by cyclic voltammetry(CV)and differential pulse voltammetry(DPV).A novel electrochemical quantitative analysis for the determination of the concentration of terbutaline was established by optimizing the concentration of ascorbic acid.The results showed that in pH=7.0,20 mmol/L Tris-HCl buffer solution,the carbon fiber electrode exhibited excellent stability toward terbutaline with the 1 × 10^-4 mol/L concentration of co-existing ascorbic acid.Under the optimal experimental conditions,the oxidation peak current was proportional to the concentration of DA in the range from 1×10^-6-1×10^-4 mol/L with a correlation coefficient of R^2 = 0.994 2.The detection limit was 2.202×10^-10 mol/L(S / N =3).In brief,the carbon fiber electrode has the advantage of simple preparation,good reproducibility and high stability,thus,it can be widely applied to highly sensitive analysis of terbutaline in biological samples.

关 键 词：碳纤维超微电极 特布他林 抗坏血酸 差分脉冲伏安法 

分 类 号：R917[医药卫生—药物分析学]