丁苯酞对脑缺血再灌注大鼠脑红蛋白和14-3-3γ蛋白的影响  

Effect of butylphthalide on the levels of Ngb and 14-3-3γ in rats with middle cerebral artery ischemia-reperfusion

作　　者：李吕力[1] 韦俊杰[1] 李晓峰[1] 李燕华[1] 张丽香[1] 陈渊[1] 封浑 肖继东[1] 陈志[1] 范秉林[1] LI Lü-Li;WEI Jun-Jie;LI Xiao-Feng(Department of Neurology,Guangxi Zhuang Autonomous Region People's Hospital,Nanning 530021,Guangxi,China)

机构地区：[1]广西壮族自治区人民医院神经内科,广西南宁530021

出　　处：《中国老年学杂志》2019年第10期2446-2448,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金(81460192);广西自然科学基金项目(2011GXNSFA018185)

摘　　要：目的探讨丁苯酞对脑缺血再灌注大鼠脑组织和血清脑红蛋白(Ngb)和14-3-3γ蛋白水平的影响。方法将30只雄性SD大鼠随机分为假手术组、缺血再灌注组和丁苯酞组,建立大鼠大脑中动脉缺血再灌注模型;24 h后采集大脑和血清,苏木素-伊红(HE)染色观察梗死灶周围的细胞损伤,采用免疫组化、酶联免疫吸附试验(ELISA)分别检测大脑和血清Ngb和14-3-3γ水平。结果 HE染色显示丁苯酞组梗死灶周围细胞损害较缺血再灌注组显著减轻,缺血再灌注组脑组织和血清14-3-3γ和Ngb表达均显著高于假手术组(P<0.05),而丁苯酞组脑组织和血清14-3-3γ和Ngb水平均显著高于缺血再灌注组(P<0.05)。结论丁苯酞减轻脑缺血再灌注大鼠脑梗死灶周围再灌注损害,其作用机制之一是上调脑组织和血清14-3-3γ和Ngb水平。Objective To explore the effect of butylphthalide on the neuroglobin(Ngb) and 14-3-3γ levels of cerebra and serum in rats with middle cerebral artery ischemia-reperfusion. Methods 30 male SD rats were randomly divided into sham operation, ischemia-reperfusion and butylphthalide-pretreated groups. The cerebral ischemia-reperfusion rat model was established in ischemia-reperfusion and butylphthalide-pretreated group. 24 hours later, the brain and serum were collected and the expressions of Ngb and 14-3-3γ were detected by ELISA and immunohistochemistry. Results The Ngb and 14-3-3γ levels of cerebra and serum were higher in ischemia-reperfusion group compared to those of sham operation group( P <0.05), while Ngb and 14-3-3γ expressions were higher in butylphthalide-pretreated group than those in ischemia-reperfusion group( P <0.05). Conclusions Butylphthalide could protect against middle cerebral artery ischemia-reperfusion in rats through upregulating production of Ngb and 14-3-3γ.

关 键 词：丁苯酞 脑缺血再灌注 神经保护 脑红蛋白 14-3-3γ蛋白 

分 类 号：R743.3[医药卫生—神经病学与精神病学]