Bmi-1-siRNA对肺腺癌A549细胞迁移和转移的影响  

The Effects of Bmi-1-siRNA on the Migration and Metastasis of Lung Adenocarcinoma A549 Cell

作　　者：刘奔 刘丹丹 张凯 郑翔宇[3] 赵宝霞[3] 孟秀香[3] LIU Ben;LIU Dandan;ZHANG Kai;ZHENG Xiangyu;ZHAO Baoxia;MENG Xiuxiang(1.School of Pharmacy and Medical Technology,Putian University,Putian Fujian 351100,China;Laboratory Center for diagnostics,Dalian Medical University,Dalian Liaoning 116044,China;College of Laboratory Medicine,Dalian Medical University,Dalian Liaoning 116044,China)

机构地区：[1]莆田学院药学与医学技术学院,福建莆田351100 [2]大连医科大学诊断学实验中心,辽宁大连116044 [3]大连医科大学检验医学院,辽宁大连116044

出　　处：《莆田学院学报》2019年第2期16-20,共5页Journal of putian University

基　　金：辽宁省科技厅自然科学基金资助项目(20072169)

摘　　要：观察Bmi-1基因沉默对A549细胞体外迁移和体内转移的影响。设计针对Bmi-1的小干扰RNA(siRNA)序列作为靶序列构建重组逆转录病毒siRNA表达载体并将其转染入肺腺癌A549细胞;应用RT-PCR和Western Blot方法检测对Bmi-1基因的沉默效果;应用划痕修复和Transwell方法检测Bmi-1-siRNA对A549细胞体外迁移的影响;通过裸鼠尾输入各组细胞,观察Bmi-1-siRNA对A549细胞在裸鼠体内转移能力的影响。结果显示:沉默Bmi-1基因的表达能够抑制A549细胞的体内外迁移能力,同时降低VEGF的分泌。Bmi-1-siRNA能抑制肺腺癌A549细胞的转移能力,VEGF可能参与其中。The effect of Bmi-1 knockdown on migration in vitro and metastasis in vivo was observed.The most effective sequence was chosen as a target sequence, then transfected into cells.The expression of siRNA and protein of Bmi-1 in lung adenocarcinoma A549 cells were assessed using RT-PCR and Western Blot respectively.The scratch repair and Trans-well assay were used to examine cell migration capacity.The potency of metastasis was observed in nude mouse through direct tail vein injected of A549 cells respectively.The results showed that Bmi-1-mRNA and protein levels all significantly reduced in A549-Bmi-1-siRNA cells, compared to A549-ctr and A549-wt cells.The siRNA-mediated knockdown of Bmi-1 significantly reduced metastasis of A549 cells involved VEGF.

关 键 词：BMI-1基因 迁移 转移 小干扰RNA 肺腺癌 

分 类 号：R734.2[医药卫生—肿瘤]