结肠癌组织中微RNA-26b和Unc-51自噬激活激酶-2表达水平与临床病理特征及预后的关系  

Expression levels of miR-26b and ULK2 in colon cancer tissues: their relationship with clinicopathological features and prognosis

作　　者：袁世超[1] 吴贵阳[1] 徐丹红[1] Yuan Shichao;Wu Guiyang;Xu Danhong(Department of Gastrointestinal Surgery,TaizhouMunicipal Hospital,Zhejiang 318000,China)

机构地区：[1]浙江省台州市立医院胃肠外科,318000

出　　处：《中国药物与临床》2019年第10期1589-1591,共3页Chinese Remedies & Clinics

基　　金：浙江省医药卫生科技计划项目(2018KY905)

摘　　要：目的探讨结肠癌组织中微RNA-26b和Unc-51自噬激活激酶-2(ULK2)表达水平与临床病理特征及预后的关系。方法本院结肠癌患者150例,实时荧光逆转录法及免疫组织化学法测定mi R-26b和ULK2在结肠癌组织及正常结肠组织中的表达水平。结果结肠癌组mi R-26b、ULK2 mRNA表达水平、蛋白表达评分、蛋白表达阳性率低于癌旁组(P<0.01);mi R-26b、ULK2蛋白表达与TMN分期、病理学分级、复发、浸润深度、淋巴血管间隙浸润、肿瘤最大径、淋巴结转移相关性明显,且TMN分期越高、病理学分期越高、有复发、浸润深度越深、有淋巴血管间隙浸润、肿瘤最大径≥5 cm、有淋巴结转移,mi R-26b、 ULK2蛋白阳性表达率越低(P<0.01);mi R-26b、ULK2阳性组3年生存率及生存期均明显高于mi R-26b、ULK2阴性组(P<0.01)。结论结肠癌组织mi R-26b、ULK2表达降低;mi R-26b、ULK2在结肠癌的发生发展过程中起抑制作用;Mi R-26b、ULK2高表达的结肠癌患者能获得较好的预后。Objective To investigate the correlation between miR-26 b and ULK2 expression in colon cancer tissues with clinicopathological features and prognosis. Methods A total of 150 patients with colon cancer were enrolled in this study. The expression levels of miR-26 b and ULK2 in colon cancer tissues and normal colon tissues were determined by real-time fluorescence reverse transcription and immunohistochemistry. Results The expression levels of miR-26 b and ULK2 mRNA and protein expression score and positive rates of protein expression were lower in colon cancer tissues than those in paracancerous tissues(P<0.01). The expression of miR-26 b and ULK2 proteins was significantly correlated with TMN stages, pathological grades, recurrence, depth of invasion, lymphovascular space infiltration, maximum tumor diameter and lymph node metastasis. Higher TMN stages, higher pathological grades, re-currence, deeper infiltration, infiltration of lymphovascular space, maximum tumor diameter ≥ 5 cm, and lymph node metastasis was associated with lower positive rate of miR-26 b and ULK2 protein expression(P<0.01). Significantly higher 3-year survival rate and longer length of survival time were noted in patients with positive miR-26 b and ULK2 than in those with negative miR-26 b and ULK2 expression(P<0.01). Conclusion The expression of miR-26 b and ULK2 in colon cancer tissues is down-regulated. Mi R-26 b and ULK2 play an inhibitory role in the development of colon cancer. More favorable prognosis may been seen in colon cancer patients with high expression of miR-26 b and ULK2.

关 键 词：结肠肿瘤 微RNA-26b Unc-51自噬激活激酶-2 

分 类 号：R735.35[医药卫生—肿瘤]