神经胶质瘤组织长链非编码RNA ANRIL表达水平及其临床意义  

Expression of long noncoding RNA ANRIL and its clinical significance in glioma tissues

作　　者：宋千 庞宏刚 祁磊[1] 梁晨 王拓[1] 王伟[1] 李瑞春[1] SONG Qian;PANG Hong-gang;QI Lei;LIANG Chen;WANG Tuo;WANG Wei;LI Rui-chun(First Affiliated Hospital of Xi'an Jiaotong University ,Xi'an 710061 ,P. R. China)

机构地区：[1]西安交通大学第一附属医院神经外科,陕西西安710061 [2]西安交通大学第一附属医院周围血管科,陕西西安710061

出　　处：《中华肿瘤防治杂志》2019年第8期535-538,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：陕西省自然科学基金面上项目(2015JM8398)

摘　　要：目的异常表达的长链非编码RNA(long noncoding RNA,lncRNA)在恶性肿瘤的发生和发展过程中发挥重要的调节作用。lncRNA-ANRIL被报道参与多种肿瘤的恶性进展,包括胶质瘤。本研究旨在探讨lncRNA-ANRIL在神经胶质瘤组织中的表达,及其与胶质瘤患者临床预后的相关性。方法选取西安交通大学第一附属医院神经外科2010-01-31-2013-01-31收治并行手术切除的71例神经胶质瘤患者,应用qRT-PCR法检测术中切除的瘤组织及其癌旁正常脑组织中lncRNA-ANRIL表达水平,并进一步分析lncRNA-ANRIL表达高低与胶质瘤患者临床病理学参数及其临床预后之间的关系。结果 lncRNA-ANRIL在神经胶质瘤组织(1.00±0.30)中呈高表达,其表达水平显著高于癌旁正常脑组织(3.25±0.63),t=3.227,P=0.009 1。lncRNA-ANRIL表达水平的高低与病理分级(χ2=19.016,P<0.001)、KPS评分(χ2=9.787,P=0.002)以及是否复发(χ2=11.424,P=0.001)具有关联性,而与患者年龄、性别、肿瘤数目和肿瘤直径均无关。lncRNA-ANRIL低表达组患者的术后生存率显著高于lncRNA-ANRIL高表达组,差异有统计学意义,χ2=6.834,P=0.008 9。病理级别的增高、KPS评分<80、术后复发以及lncRNA-ANRIL高表达均是影响胶质瘤患者临床预后的危险因素,P<0.05。结论神经胶质瘤组织中lncRNA-ANRIL表达水平显著升高,高表达水平的lncRNA-ANRIL与胶质瘤患者不良预后密切相关,lncRNA-ANRIL可作为预测胶质瘤患者临床预后的新肿瘤标志物。OBJECTIVE Dysregulated long noncoding RNA(lncRNA)plays critical roles in tumorigenesis and cancer progression.lncRNA-ANRIL has been showed to be involved in the initiation and development of several cancers,including glioma.However,the expression of lncRNA-ANRIL in glioma tissue and its correlation with the clinical prognosis of glioma patients were not reported.METHODS In this study,a total of 71 glioma patients admitted by neurosurgery department of the First Affiliated Hospital of Xi’an Jiaotong University from January 31,2010 to January 31,2013 for concurrent surgical resection were selected.The expression level of lncRNA-ANRIL in tumor tissues and normal brain tissues adjacent to cancer was detected by qRT-PCR.RESULTS The expression of lncRNA-ANRIL in glioma tissue(1.00±0.30)was significantly higher than that of normal brain tissue(3.25±0.63,t=3.227,P=0.009 1).The level of lncRNA-ANRIL expression was correlated with pathological grading,KPS score and recurrence,but not with age,sex,tumor number,tumor diameter,etc.The postoperative survival rate of patients in the lncRNA-ANRIL low expression group was significantly higher than that in the lncRNA-ANRIL high expression group,and the difference was statistically significant(P=0.008 9).The advance of pathological level,KPS<80,postoperative recurrence and high lncRNA-ANRIL expression were the risk factors(P<0.05)that affected the clinical prognosis of glioma patients.CONCLUSIONS The expression level of lncRNA-ANRIL in glioma tissues is significantly elevated,and the high expression level of lncRNAANRIL is closely related to the adverse prognosis of glioma patients.lncRNA-ANRIL can be used as a new tumor marker to predict the clinical prognosis of glioma patients.

关 键 词：神经胶质瘤 长链非编码RNAANRIL 病理学临床预后 

分 类 号：R739.41[医药卫生—肿瘤]