肉苁蓉多糖闪式提取工艺及其抗糖基化分析  

Optimization of flash extraction of polysaccharides from Cistanche deserticola and its antiglycosylation activity

作　　者：张喜峰[1,2] 邓猛猛 罗光宏 杨生辉 王丹霞 ZHANG Xi-feng;DENG Meng-meng;LUO Guang-hong;YANG Sheng-hui;WANG Dan-xia(College of Agriculture and Biotechnology,Hexi University,Zhangye 734000,China;Key Laboratory of Hexi Corridor Resources Utilization of Gansu,Zhangye 734000,China;Gansu Engineering Research Center for Microalgae,Hexi University,Zhangye 734000,China)

机构地区：[1]河西学院农业与生物技术学院,甘肃张掖734000 [2]甘肃省河西走廊特色资源利用重点实验室,甘肃张掖734000 [3]河西学院甘肃省微藻工程技术研究中心,甘肃张掖734000

出　　处：《甘肃农业大学学报》2019年第2期193-198,204,共7页Journal of Gansu Agricultural University

基　　金：甘肃省中小企业创新基金资助项目(1110FCCG112)

摘　　要：【目的】确定肉苁蓉多糖的闪式提取工艺条件并分析其抗糖基化活性.【方法】采用单因素和响应面试验优化料液比、转速以及提取时间对多糖得率的影响,及其对赖氨酸/葡萄糖模拟体系中形成的晚期糖基化终产物(AGEs)的抑制效果.【结果】当料液比1∶53 (g/mL)、提取转速5 387 r/min、提取时间60 s时,多糖得率可达12.35%.制备多糖和阳性对照氨基胍(AG)对模拟体系中AGEs半数抑制浓度IC_(50)分别为0.438和0.341 mg/mL.【结论】采用闪式提取肉苁蓉多糖方法简单可行,回收率高,其多糖具有一定糖基化抑制效果.【Objective】To optimize the extraction process of polysaccharides from Cistanche deserticola(CDPs)using flash extraction,and to analyze antiglycosylation activity.【Method】The effect of different parameters ranging from solid-liquid ratio,voltage,and extraction time on the yield of CDPs were optimized through single factor experiments and response surface experiments.The effects of CDPs on the advanced glycation end products(AGEs)in Lysine/glucose models were also investigated.【Result】The optimal extraction conditions with a yield of 12.35% for extraction of CDPs were the ratio of solid to liquid of 1∶53(g/mL),a rotating speed of 5387 r/min,and the extraction time for 1 min.The IC50 values of CDPs and AG against AGEs were 0.438 and 0.341 mg/mL,respectively.【Conclusion】As a simple and efficient technique,it can be used to extract CDPs,which possess the inhibitory effects of antiglycosylation activity.

关 键 词：肉苁蓉 闪式提取 晚期糖基化终产物 

分 类 号：TS201[轻工技术与工程—食品科学]