EBV基因组测序的研究进展  

Advances in sequencing of the Epstein-Barr Virus genome

作　　者：赖永静 夏巍 杜龙[1] 王梦琳[1] 冯清源 戢克铜 唐翔龙[1] 唐安洲[1,2] LAI Yong jing;XIA Wei;DU Long;WANG Mcng-ling;FENG Qing-yuan;JI Ke-tong;TANG Xiang-long;TANG An-zhou(The First Hospital Afjilluted with Guangxi Medical University,Nanning,Guangxi,China 530000;Key laboratory for Early Prevention and Treatment of Highly Endemic Tumors,Ministry of Education)

机构地区：[1]广西医科大学第一附属医院,广西南宁530000 [2]广西区域性高发肿瘤早期防治研究教育部重点实验室

出　　处：《中国病原生物学杂志》2019年第4期485-490,共6页Journal of Pathogen Biology

基　　金：国家自然科学基金项目(No.81560486);广西科技攻关项目(桂科攻14124003-2)

摘　　要：EB病毒在全世界范围内广泛分布,科学家们发现EB病毒与人类单核细胞增多症及多种恶性肿瘤相关。EB病毒的地理分布和EB病毒相关疾病的地方病发病率特点促使人们研究是否有不同的EB病毒株参与疾病的发展。第一代测序技术的低效率和高成本限制了全基因组EBV的测序,随着第二代测序技术(NGS)的发展,越来越多的EB病毒基因组序列发表。这将有助于更好地理解EBV在不同地理区域的遗传变异,并在特定疾病中发现可能的致病变异。本文概述了已发表的EBV基因组的概况及用于生成这些序列的测序技术和生物信息学分析,探讨EBV全基因组未来的研究方向。Epstein-Barr virus(EBV)is widely distributed all over the world.Scientists have found that EBV is associated with infectious mononucleosis and various malignant tumors.Certain EBV-associated diseases,such as Burkitt lymphomas and nasopharyngeal carcinomas,are endemic and exhibit skewed geographic distribution worldwide.The geographical distribution of EBV strains and the incidence of EBV-associated diseases have prompted studies of whether distinct strains of EBV are involved.Sequencing of EBV has been limited by the inefficiency and cost of first-generation sequencing technology.Recent advances in sequencing technology have enabled high-throughput sequencing of the EBV genome.With the development of next-generation sequencing(NGS),a number of EBV genomes have been published.These genomes were sequenced using different approaches such as enrichment of EBV DNA or no enrichment.This review provides an overview of the EBV genomes published and a description of the sequencing technology and bioinformatic analyses used to examine EBV sequences.This review also describes recent advances and potential future directions for EBV sequencing.

关 键 词：EB病毒 全基因组测序 Sanger测序 NGS 综述 

分 类 号：R373.11[医药卫生—病原生物学]