Sensitivity assessment of Phytophthora nicotianae to fungicides of fluazinam and its mixture with carboxylic acid amide  

作　　者：MU Wenjun LI Qi CHEN Xiuhua LI Haijiang DONG Kunle WANG Jianwei ZHANG Youwu FENG Xiaohu ZHANG Zhigao WANG Libing SONG Jizhen 

机构地区：[1]Key Laboratory of Eco-environment and Leaf Tobacco Quality, Zhengzhou Tobacco Research Instilule of CNTC. No.2 Fengyang Street, Zhengzhou 450001,China [2]Henan Tobacco Company, No. 7 Zhengqi Street, Zhengzhou 450008, China [3]Pingdingshan Branch of Henan Provincial Tobacco Company, No.263 Jianshe Road, Pingdingshan 467000, Henan, China [4]Luoyang Branch of Henan Provincial Tobacco Company, No.246 Kaiyuan Avenue, Luoyang 471026,Henan, China [5]Fuzhou Branch of Jiangxi Provincial Tobacco Company, No. 1666 Yingbin Avenue, Fuzhou 344000, Jiangxi, China

出　　处：《烟草科技》2018年第A01期1-7,共7页Tobacco Science & Technology

摘　　要：Tobacco black shank caused by Phytophthora parasitica var.nicotianae(Breda de Hean)is one of the most serious diseases in tobacco growing,and few effective fungicides are available currently.The purpose of this study was to assess the feasibility of using fluazinam to treat tobacco black shank disease.In vitro toxicities of fluazinam to 62 isolates of P.nicotianae were determined by mycelial growth rate method.The EC50 values ranged from 0.294 6 to 2.389 9 μg/mL with a mean of 1.084 0 μg/mL.The sensitivity distribution was unimodal,and there was no resistant isolate in P.nicotianae population.The carboxylic acid amide(CAA)fungicides were single-site in action and activity to oomycetes.The synergistic interactions were tested by Wadley method between fluazinam and dimethomorph,flumorph,mandipropamid,respectively.The mixture containing dimethomoph:Huazinam at 1:2 ratio showed additive actions,with the synergy ratio of 1.2.The mixture containing flumorph and fluazinam at 1:5 ratio had the highest synergy ratio(SR 2.07).Mandipropamid and fluazinam had an additive interaction and the proportion of 2:1 had the lowest EC50 value(0.020 7 μg/mL).This study demonstrated the sensitivity of P.nicotianae to fluazinam,and provided the experimental foundation to use fluazinam for tobacco black shank management.

关 键 词：PHYTOPHTHORA nicotianae FLUAZINAM DIMETHOMORPH FLUMORPH MANDIPROPAMID Fungicide MIXTURE 

分 类 号：TS[轻工技术与工程]