反复短暂缺血可抑制坐骨神经损伤模型大鼠失神经支配的腓肠肌萎缩  

Repeated transient ischemia inhibits denervated gastrocnemius atrophy in rat models of sciatic nerve injury

作　　者：周晓彬[1,5] 王东 林朋朝[1] 高伟静 姬艳林 祁欢 李静 周君琳[2] Zhou Xiaobin;Wang Dong;Lin Pengzhao;Gao Weijing;Ji Yanlin;Qi Huan;Li Jing;Zhou Junlin(Third Department of Traumatology,Shijiazhuang Third Hospital,Shijiazhuang 050000,Hebei Province,China;Department of Orthopedic Traumatology,Beijing Chao-Yang Hospital,Capital Medical University,Beijing 100008,China;Second Department of Traumatology,Shijiazhuang Third Hospital,Shijiazhuang 050000,Hebei Province,China;Department of Traditional Chinese Medicine,Hebei Provincial Geriatric Hospital,Shijiazhuang 050000,Hebei Province,China)

机构地区：[1]石家庄市第三医院创伤三科,河北省石家庄市050000 [2]首都医科大学附属北京朝阳医院创伤骨科,北京市100008 [3]石家庄市第三医院创伤二科,河北省石家庄市050000 [4]河北省老年病医院中医科,河北省石家庄市050000 [5]首都医科大学

出　　处：《中国组织工程研究》2019年第23期3667-3672,共6页Chinese Journal of Tissue Engineering Research

基　　金：河北省医学科学研究重点课题计划(20181060),项目负责人:周晓彬;北京市科学技术委员会(Z161100000116080),项目负责人:周君琳~~

摘　　要：背景:既往实验表明反复短暂缺血可以诱导肌肉的缺血耐受,达到营养靶器官的作用。目的:探讨反复短暂缺血对坐骨神经损伤模型大鼠失神经支配腓肠肌细胞萎缩的抑制作用。方法:60只8周龄SD大鼠,由北京维通利华动物技术有限公司提供,实验过程得到首都医科大学动物实验伦理委员会批准(伦理号AEEI-2017-055)。随机将大鼠分为3组,实验组和对照组大鼠均建立坐骨神经损伤模型,实验组患侧下肢给予每天1次3×(10min缺血/10min再灌注);对照组:吻合神经后不给予反复短暂缺血处理;假手术组:未损伤神经,给予患侧肢体每天1次3×(10min缺血/10min再灌注)。比较2周及4周时3组间腓肠肌湿质量维持率,腓肠肌苏木精-伊红染色,坐骨神经电生理及ELISA测定腓肠肌内血管内皮生长因子的含量。结果与结论:①电生理显示4周时实验组与对照组均未检测到腓肠肌的动作电位及潜伏期;②腓肠肌湿质量维持率检测结果显示实验组较对照组明显增加(P<0.05),假手术组优于实验组和对照组(P<0.01);③腓肠肌纤维横截面积结果显示实验组肌纤维化较对照组明显减轻(P<0.05);④ELISA检测结果显示实验组及假手术组腓肠肌内血管内皮生长因子含量均显著高于对照组(P<0.05);⑤结果说明,神经损伤后4周内,反复短暂缺血可以有效提高腓肠肌内源性血管内皮生长因子的表达,从而发挥内源性血管内皮生长因子抑制腓肠肌萎缩的作用。BACKGROUND:Preliminary study has shown that repeated transient ischemia may induce muscle ischemia tolerance to enrich target organs.OBJECTIVE:To investigate the inhibitory effect of repeated transient ischemia on atrophy of denervated gastrocnemius muscle cells in a rat model of sciatic nerve injury.METHODS:Sixty Sprague-Dawley rats aged 8 weeks were provided by Beijing Weitong Lihua Animal Technology Co.,Ltd.,and the study was approved by the Laboratory Animal Ethics Committee of Capital Medical University,approval number:AEEI-2017-055.The rats were randomly divided into control group(sciatic nerve injury model),experimental group(sciatic nerve injury model plus 3×(10-minute ischemia/10-minute reperfusion)and sham group [3×(10-minute ischemia/10-minute reperfusion)].The wet mass maintenance rate of gastrocnemius,hematoxylin-eosin staining of gastrocnemius,electrophysiology of sciatic nerve,and content of vascular endothelial growth factor in gastrocnemius detected by ELISA were compared among groups at 2 and 4 weeks.RESULTS AND CONCLUSION:(1)Electrophysiology showed that neither experimental nor control groups presented action potential or latency at 4 weeks.(2)The wet mass maintenance rate of gastrocnemius in the experimental group was significantly increased compared with the control group(P<0.05),and the sham group was significantly superior to the experimental and control groups(P<0.01).(3)The cross-sectional area of gastrocnemius fibers in the experimental group was significantly decreased compared with the control group(P<0.05).(4)The content of vascular endothelial growth factor in gastrocnemius detected by ELISA in the experimental and sham groups was significantly higher than that in the control group(P<0.05).(5)These results suggest that within 4 weeks of nerve injury,the repeated transient ischemia can effectively improve the expression of endogenous vascular endothelial growth factor in gastrocnemius,thus inhibiting its atrophy.

关 键 词：反复短暂缺血 腓肠肌 血管内皮生长因子 电生理 肌萎缩 坐骨神经损伤 大鼠 

分 类 号：R446[医药卫生—诊断学] R318[医药卫生—临床医学]