子宫内膜癌组织中miR-10a及let-7i的表达及临床意义  

Expression and clinical significance of miR-10a and let-7i in endometrial carcinoma

作　　者：齐丽宁[1] 张红真[2] 魏旭静 郭艳蒲 翟爱丽[1] 王琪[1] 李淼[1] 朱继红 QI Li-ning;ZHANG Hong-zhen;WEI Xu-jing;GUO Yan-pu;ZHAI Ai-li;WANG Qi;LI Miao(Department of Gynaecology and Obstetrics,The First Central hospital of Baoding,Baoding 071000,Hebei,China;Department of Gynaecology and Obstetrics,The First Hospital of Hebei Medical University,Shijiazhuang 050011,Hebei,China)

机构地区：[1]保定市第一中心医院妇产科,河北保定071000 [2]河北医科大学第一医院妇产科,石家庄050011 [3]不详

出　　处：《肿瘤代谢与营养电子杂志》2019年第1期75-78,共4页Electronic Journal of Metabolism and Nutrition of Cancer

摘　　要：目的探讨miR-10a及let-7i在子宫内膜癌组织中的表达及意义。方法选取子宫内膜癌组织40例、子宫内膜不典型增生组织20例及正常子宫内膜组织30例,采用多聚腺苷酸加尾实时荧光定量逆转录聚合酶链反应[poly(A)-RT-qPCR]测定miR-10a及let-7i在三种子宫内膜组织中的表达,并将结果与临床及病理资料进行分析。结果miR-10a在子宫内膜癌组织中高表达(高于正常及不典型增生的子宫内膜组织,P<0.01);在病理分级为3级的患者中高表达(高于1+2级者);在Ⅲ+Ⅳ期患者中高表达(高于Ⅰ+Ⅱ期者);在有淋巴结转移的患者中高表达,在深肌层浸润(浸润深度≥1/2)的患者中高表达;差异显著(P<0.05)。在不同年龄、雌激素受体(ER)及孕激素受体(PR)是否阳性的子宫内膜癌患者中其表达量差异无统计学意义(P>0.05)。let-7i在子宫内膜癌组织低表达(低于正常和不典型增生的子宫内膜组织,P<0.01)。结论在子宫内膜癌组织中miR-10a表达上调,let-7i表达下调,miR-10a可能参与并促进了子宫内膜癌的侵袭和转移。Objective To investigate the expression of miR-10a and let-7i in endometrial carcinoma tissues and its significance.Methods Forty cases of endometrial carcinoma,twenty cases of atypical hyperplasia and thirty cases of normal endometrium tissues were collected,poly(A)-RT-qPCR was employed to test the expression of miR-10a and let-7i in different endometrial tissues.The statistical analyses were performed between the expression of miR-10a and let-7i and the clinicopathological characteristics.Results The expression of miR-10a was higher in endometrial carcinoma tissues than that of normal endometrium and atypical hyperplasia tissues(P<0.01).The expression of miR-10a in patients with grade 3 of pathology was higher than that 1+2,in stageⅢ+Ⅳof endometrial carcinoma patients was higher than stageⅠ+Ⅱ,in patients with lymph node metastasis was higher than that without lymph node metastasis,in patients that myometrial invasion≥1/2 was higher than that<1/2.The expression of miR-10a in different age,whether ER-positive,PR-positive had no statistical difference(P>0.05).The expression of let-7i was lower in endometrial carcinoma tissues than that of normal endometrium and atypical hyperplasia tissues(P<0.01).Conclusions In endometrial carcinoma tissues,the expression of miR-10a was up-regulated,let-7i was down-regulated,miR-10a may promote invasion and metastasis of endometrial carcinoma.

关 键 词：子宫内膜癌 MiR-10a Let-7i 

分 类 号：R737.33[医药卫生—肿瘤]