CUG连接蛋白1在乳腺癌组织中的表达及其对细胞活力和侵袭能力的影响  

Expression of CUG-binding protein 1 in breast cancer and its effect on cell viability and invasion ability

作　　者：翟媛媛[1] 柴丽君[1] 郑英斌[1] 梁冰[1] ZHAI Yuan-yuan;CHAI Li-jun;ZHENG Ying-bin;LIANG Bing(Department of Breast Surgery,The Second Affiliated Hospital of Zhengzhou University,Zhengzhou 450014,China)

机构地区：[1]郑州大学第二附属医院乳腺科,河南郑州450014

出　　处：《中国病理生理杂志》2019年第5期825-829,共5页Chinese Journal of Pathophysiology

基　　金：河南省基础与前沿技术研究项目(No.142300410207)

摘　　要：目的:探讨CUG连接蛋白1(CUGBP1)在乳腺癌组织中的表达,以及沉默CUGBP1基因对人乳腺癌MCF-7细胞活力和侵袭能力的影响。方法:选取2015年3月～2017年9月在郑州大学第二附属医院手术治疗的乳腺癌患者96例,免疫组化法检测乳腺癌和癌旁组织中CUGBP1蛋白表达,培养MCF-7细胞并分为CUGBP1干扰序列组、对照序列组和空白组,Western blot法检测各组细胞中CUGBP1、Twist、上皮型钙黏附蛋白(E-cadherin)和波形蛋白(vimentin)的蛋白表达,MTT法检测各组细胞活力,Transwell法检测细胞侵袭能力。结果:乳腺癌组织中CUGBP1阳性表达率高于癌旁组织(χ~2=28.900,P<0.001);乳腺癌组织中CUGBP1蛋白表达在TNM分期、组织学分级和淋巴结转移差异有统计学意义(P<0.05);CUGBP1干扰序列组细胞中CUGBP1、Twist和vimentin蛋白相对表达量均低于对照序列组和空白组,而E-cadherin蛋白相对表达量均高于对照序列组和空白组(P<0.05);CUGBP1干扰序列组24 h、48 h、72 h和96 h时细胞活力均低于对照序列组和空白组(P<0.05);CUGBP1干扰序列组侵袭细胞数少于对照序列组和空白组(P<0.05)。结论:乳腺癌组织中CUGBP1蛋白呈高表达。特异性沉默乳腺癌MCF-7细胞CUGBP1基因表达,可有效抑制细胞活力及侵袭能力,其机制可能与抑制上皮-间充质转化过程有关。AIM:To investigate the expression of CUG-binding protein 1(CUGBP1)in breast cancer tissues,and to explore the effect of CUGBP1 gene silencing on the viability and invasion ability of human breast cancer MCF-7 cells.METHODS:A total of 96 cases of patients with breast cancer undergoing surgical treatment were selected in the Second Affiliated Hospital of Zhengzhou University from March 2015 to September 2017.Immunohistochemical staining was used to detect the protein expression of CUGBP1 in the breast cancer and adjacent tissues.MCF-7 cells were cultured and divided into CUGBP1 interference sequence group,control sequence group and blank group.Western blot was used to detect the protein expression of CUGBP1,Twist,E-cadherin and vimentin in the cells.The cell viability was measured by MTT assay.The cell invasion ability was detected by Transwell assay.RESULTS:The positive expression rate of CUGBP1 protein in the breast cancer tissues was higher than that in the adjacent tissues(X^2=28.900,P<0.001).The differences of CUGBP1 protein expression in the breast cancer tissues among TNM staging,histological grading and lymph node metastasis were statistically significant(P<0.05).The relative protein expression levels of CUGBP1,Twist and vimentin in CUGBP1 interference sequence group were lower than those in control sequence group and blank group,while the relative protein expression of E-cadherin was higher than that in control sequence group and blank group(P<0.05).The cell viability at 24 h,48 h,72 h and 96 h in CUGBP1 interference sequence group was lower than that in control sequence group and blank group(P<0.05).The invasive cells in CUGBP1 interference sequence group were less than those in control sequence group and blank group(P<0.05).CONCLUSION:CUGBP1 protein is highly expressed in the breast cancer tissues.Specific silencing of CUGBP1 gene expression in breast cancer MCF-7 cells effectively inhibits the cell viability and invasiveness,and its mechanism may be related to inhibiting the process of epithelial-mesenc

关 键 词：乳腺癌 CUG连接蛋白1 细胞活力 细胞侵袭 上皮-间充质转化 

分 类 号：R737.9[医药卫生—肿瘤] R730.23[医药卫生—临床医学]