FNA对短程硝化污泥菌群结构的影响  

Effect of FNA on microorganism community structures of partial nitrification sludge

作　　者：高春娣 赵楠 安冉 韩徽 张娜 彭永臻 GAO Chun-di;ZHAO Nan;AN Ran;HAN Hui;ZHANG Na;PENG Yong-zhen(National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology,College of Environmental and Energy Engineering,Beijing University of Technology,Beijing 100124,China)

机构地区：[1]北京工业大学环境与能源工程学院城镇污水深度处理与资源化利用技术国家工程实验室,北京100124

出　　处：《中国环境科学》2019年第5期1977-1984,共8页China Environmental Science

基　　金：国家自然科学基金资助项目(51478012);北京市科技重大专项(Z181100005318001)

摘　　要：在SBR反应器增加游离亚硝酸(FNA)预处理单元,投加浓度为1.2mg HNO_2-N/L的FNA进行缺氧搅拌4.5h,连续处理3d,考察短程硝化污泥中FNA对氨氧化菌(AOB),丝状菌和微生物菌群结构的影响.研究表明,FNA对AOB有短时抑制作用,并能够抑制优势丝状菌Candidatus_Microthrix(微丝菌属)和Cytophagaceae(噬纤维菌)的增殖,分别由5.1%和1.1%下降到0.78%和几乎不可见.SVI从281mL/g降低到100mL/g左右.NAR能够维持在90%左右,短程硝化不受到破坏.高通量结果显示,FNA处理后微生物菌群结构多样性与丰度出现下降,但Thauera(陶厄氏菌属)和Ottowia出现了增殖,分别增加到5.58%和7.82%,同步硝化反硝化(SND)作用明显,这使得即便只有短程硝化,总氮去除率依然能达到60%以上.The long-term effects on ammonia-oxidizing bacteria(AOB), filamentous bacteria and microorganism community structures in partial nitrification sludge was investigated by adding a pretreatment unit of free nitrous acid(FNA) in the sequence bath reactor(SBR) for three days at 1.2 mg HNO2-N/L for 4.5. The results showed that FNA had the short-time effect on AOB,Candidatus_Microthrix and Cytophagaceae of the dominant filamentous bacteria were also decreased from 5.1% and 1.1% to 0.78% and almost invisible. Sludge volume index(SVI) maintained at 110 mL/g dropping from 281 mL/g, and the nitrite accumulation rate(NAR) was kept at around 90%, indicating the partial nitrification was not undermined. Furthermore, High-throughput sequencing results showed that the diversity and uniformity of microorganism community decreased. However, the proliferation of Thauera and Ottowia increased to 5.58% and 7.82%. Simultaneous nitrification and denitrification(SND) had significant effects, and the total nitrogen removal rate was nevertheless maintained more than 60% even only with partial nitrification.

关 键 词：短程硝化 污泥膨胀 污泥沉降性能 丝状菌 AOB 微生物菌群结构 SND 

分 类 号：X172[环境科学与工程—环境科学]