茶树体胚APX基因克隆及其在体胚发生过程中的表达与酶活性分析  

Cloning of Ascorbate Peroxidase Gene and Analysis of Its Gene Expression and Enzyme Activity of Somatic Embryogenesis in Camellia sinensis

作　　者：郭玉琼[1] 黄道斌 李小桢 朱晨[1,2] 张舒婷 傅海峰[1] 周承哲 欧阳明秋 陈兰 赖钟雄 GUO Yuqiong;HUANG Daobin;LI Xiaozhen;ZHU Chen;ZHANG Shuting;FU Haifeng;ZHOU Chengzhe;OUYANG Mingqiu;CHEN Lan;LAI Zhongxiong(College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;Institute of Horticultural Biotechnology, Fujian Agriculture and Foresty University, Fuzhou, Fujian 350002, China)

机构地区：[1]福建农林大学园艺学院,福建福州350002 [2]福建农林大学园艺植物生物工程研究所,福建福州350002

出　　处：《热带作物学报》2019年第5期922-931,共10页Chinese Journal of Tropical Crops

基　　金：福建省自然科学基金项目(No.2018J01701);福建农林大学科技创新专项基金项目(No.CXZX2017164;No.CXZX2018069);福建省高原学科建设经费项目(No.102/71201801101)

摘　　要：以铁观音茶树体胚为材料,克隆了茶树抗坏血酸过氧化物酶基因CsAPX(Genbank登录号为MG799534.1)。CsAPX基因包含一个长度为753 bp的开放阅读框(ORF),共编码250个氨基酸,并对其进行相关生物信息学分析。APX酶活性测定结果表明在球形胚至子叶胚阶段APX酶活性呈现下降趋势,并在子叶胚阶段到达最低值,随着体胚进一步的分化,在子叶胚、成熟胚和萌发胚阶段,茶树APX酶活性不断上升,总体呈"先降后升"的趋势。在体胚发生过程中CsAPX基因的表达分析显示,CsAPX基因在茶树体胚发生的5个阶段均存在显著差异表达,总体呈"升—降—升"的趋势,其中在萌发胚阶段的CsAPX基因的表达水平显著高于其他4个阶段,推测CsAPX基因在体胚发生过程中的萌发胚阶段发挥着重要的作用。The ascorbate peroxidase gene CsAPX(GenBank accession number: MG799534.1) was cloned from the somatic embryo of Camellia sinensis cultivar ‘Tieguanyin’. The CsAPX gene contained an open reading frame(ORF) of753 bp in length, which would encode a total of 250 amino acids, and bioinformatics analysis about CsAPX gene was also employed. The total APX activity was measured during the whole somatic embryogenesis and the results showed that the total APX enzyme activity presented a downward trend in the globular embryo to cotyledonary embryo stage,and reached the lowest value in the cotyledon embryo stage. With the further differentiation of the embryo cells, the total APX enzyme activity increased continuously during the cotyledonary embryo stage, the embryo maturation stage and the germinating stage, and showed an overall trend of "decreasing first and then rising". The expression of CsAPX was significantly different between the 5 stages of the somatic embryogenesis, showing an overall trend of "up-downup" with a significantly higher expression level in the germinating stage than in the other 4 embryo stages. It was speculated that CsAPX played an important role in the germinating stage.

关 键 词：茶树体胚 抗坏血酸过氧化物酶 基因克隆 表达分析 酶活性分析 

分 类 号：S571.1[农业科学—茶叶生产加工]