MALAT-1和HIF-1α在三阴型乳腺癌中表达及临床意义  

Expression and clinical significance of MALAT-1 and HIF-1α in triple negative breast cancer

作　　者：唐静[1] 贾昱娴[1] 农丽[1] 覃芳卉[1] 钟武宁 谭爱花[1] 刘燕[1] 王洪学[1] 王涵[1] 谢伟敏[1] 陆永奎[1] 周文献[1] TANG Jing;JIA Yuxian;NONG Li;QIN Fanghui;ZHONG Wuning;TAN Aihua;LIU Yan;WANG Hongxue;WANG Han;XIE Weimin;LU Yongkui;ZHOU Wenxian(Department of Medical Oncology of Breast and Bone Soft Tissue Tumor,Affiliated Tumor Hospital of Guangxi Medical University,Nanning,Guangxi 530021,China)

机构地区：[1]广西医科大学附属肿瘤医院乳腺及骨软组织肿瘤内科,南宁530021

出　　处：《重庆医学》2019年第10期1642-1645,共4页Chongqing medicine

基　　金：国家自然科学基金项目(81602315);广西壮族自治区卫生和计划生育委员会自筹经费课题(Z20170446;Z2016485);广西壮族自治区中医药民族医药自筹经费课题(GZZC16-37)

摘　　要：目的探讨肺腺癌转移相关转录本-1(MALAT-1)、低氧诱导因子-1(HIF-1)αmRNA在三阴型乳腺癌(TNBC)中的表达及临床意义。方法采用免疫组织化学SP法检测HIF-1α的蛋白表达水平;实时荧光定量聚合酶链反应(RT-PCR)检测MALAT-1、HIF-1αmRNA的表达情况,分析其与TNBC临床特征的关系。结果 MALAT-1、HIF-1αmRNA在乳腺癌组织中的表达明显高于癌旁组织(2.62±0.23 vs.2.02±0.08;129.79±15.34 vs.43.46±5.14;P<0.05),HIF-1α蛋白在TNBC组织中的阳性表达率显著高于癌旁组织(69.7%vs.45.4%,P<0.05)。癌组织中的MALAT-1、HIF-1αmRNA的表达水平与TNBC组织的组织学分化程度、浸润深度、淋巴结转移、临床分期相关(P<0.01)。结论检测MALAT-1、HIF-1αmRNA的表达水平可能对TNBC浸润转移及增殖有一定的预测意义。Objective To investigate the expression and clinical significance of metastasis-associated lung adenocarcinoma transcript 1(MALAT-1)and hypoxia inducible factor-1α(HIF-1α)in triple negative breast cancer(TNBC).Methods Immunohistochemical SP method was used to detect the expression of HIF-1αin 33 cases of TNBC tumor tissues and their corresponding paracancer tissues.HIF-α,MALAT-1 mRNA levels were measured by quantitative real-time PCR(RT-PCR).The correlation between HIF-1α,MALAT-1 levels and the clinical features of TNBC were analyzed.Results The expression of HIF-1α,MALAT-1 mRNA in breast tumor tissues was significantly higher than that in paracancer tissues in TNBC(2.62±0.23 vs.2.02±0.08;129.79±15.34 vs.43.46±5.14;P<0.05).The positive expression rate of HIF-1αin TNBC increased significantly compared with those in paracancer tissues(69.7%vs.45.4%,P<0.05).The positive expression rate of HIF-1α,MALAT-1 mRNA in TNBC patients correlated with histological grading,infiltration depth,lymph node metastasis,and clinical staging(P<0.01).Conclusion Detection of the expression level of MALAT-1 and HIF-1αmRNA may have some predictive significance for the invasion,metastasis and proliferation of TNBC.

关 键 词：乳腺肿瘤 逆转录聚合酶链反应 肺腺癌转移相关转录本-1 低氧诱导因子-1Α 

分 类 号：R733.4[医药卫生—肿瘤]