miRNA-141对胃癌细胞增殖及侵袭的抑制作用  

Inhibition of miRNA-141 on proliferation and invasion of gastric cancer cells

作　　者：张诚[1] 周超[1] ZHANG Cheng;ZHOU Chao(Department of Gastroenterology,Renji Hospital,Shanghai Jiaotong UniversitySchool of Medicine,Shanghai 200127,China)

机构地区：[1]上海交通大学医学院附属仁济医院胃肠外科,上海200127

出　　处：《重庆医学》2019年第9期1476-1479,共4页Chongqing medicine

基　　金：上海交通大学医学院科技基金项目(2016XJ026)

摘　　要：目的探讨miRNA-141对胃癌细胞增殖及侵袭的抑制作用。方法实时荧光定量PCR(realtime PCR)法检测胃癌细胞NCI-N87、SGC-7901、MGC-803、MKN-45、HGC-27、BGC-823及正常人胃黏膜上皮细胞株GES-1中miRNA-141表达,脂质体将miRNA-141mimics和miRNA-141NC转入胃癌细胞中,48h后,real-time PCR法检测miRNA-141表达,甲基偶氮唑蓝(MTT)法检测细胞活力,膜联蛋白V-异硫氰酸荧光素/碘化丙啶(Annexin V-FITC/PI)流式双染检测细胞凋亡,Transwell实验检测细胞侵袭能力,Western blot检测增殖细胞核抗原(PCNA)、c-myc、基质金属蛋白酶(MMP)-2、MMP-9表达及磷脂酰肌醇-3-激酶/蛋白激酶B(PI3K/AKT)信号通路激活情况。结果胃癌细胞NCI-N87、SGC-7901、MGC-803、MKN-45、HGC-27、BGC-823中miRNA-141相对表达水平(2.05±0.20、1.76±0.17、1.04±0.10、1.23±0.12、0.89±0.09、0.45±0.04)明显低于GES-1中(2.87±0.30)的表达水平(P<0.01)。与miRNA-141NC组比较,miRNA-141mimics组miRNA-141表达水平明显升高、细胞活力下降、细胞凋亡率升高、细胞侵袭能力降低,PCNA、c-myc、MMP-2、MMP-9、PI3K及磷酸化AKT(p-AKT)表达水平下调,差异均有统计学意义(P<0.01)。结论上调BGC-823胃癌细胞中miRNA-141表达水平后能明显抑制癌细胞增殖与侵袭,可能与抑制阻断PI3K/AKT信号通路有关。Objective To explore the inhibition of miRNA-141 on proliferation and invasion of gastric cancer cells.Methods Expression of miRNA-141 in gastric cancer cell NCI-N87,SGC-7901,MGC-803,MKN-45,HGC-27,BGC-823 and GES-1 in normal human gastric epithelial cell was examined by real-time PCR.miRNA-141 mimics and miRNA-141 NC were transfected into gastric cancer cells by liposome.Cell viability was measured by MTT assay.Cell apoptosis was measured by Annexin V-FITC/PI flow cytometry.Cell invasion was measured by transwell method.The expression of proliferating cell nuclear antigen(PCNA),c-myc,matrix metalloproteinase-2(MMP-2),matrix metalloproteinase-9(MMP-9),the activation of phosphatidylinositol 3 kinase/protein kinase B(PI3 K/AKT)was measured by Western blot.Results The expression of miRNA-141 in NCI-H460,NCI-H1975,NCI-H446,A549,NCI-H1299,NCI-H157 cell(2.05±0.20,1.76±0.17,1.04±0.10,1.23±0.12,0.89±0.09,0.45±0.04,respectively)was lower than that in GES-1 cell(2.87±0.30).In the miRNA-141 mimics group,the cell viability reduced,the cell apoptotic rate increased,and the cell invasion ability decreased when compared with the miRNA-141 NC group(P<0.01).The expression of PCNA,c-myc,MMP-2,MMP-9,PI3 K,p-AKT was down-regulated in the miRNA-141 mimics group(P<0.01).Conclusion The up-regulation of miRNA-141 in gastric cancer cells could inhibit the cell proliferation and invasion,which might be related to the inhibition of PI3 K/AKT signaling pathway.

关 键 词：miRNA-141 胃肿瘤 细胞增殖 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]