不同色光对蛇足石杉离体叶状体产HupA及内源激素水平的影响  

The Effects of Different Colored Light on HupA Accumulation and Endogenous Hormone Content Produced by the Thallus of Huperzia serrata in vitro

作　　者：黄倩 涂艺声[1] 余晓 袁慧慧 HUANG Qian;TU Yisheng;YU Xiao;YUAN Huihui(College of Life Science,Jiangxi Normal University,Nanchang Jiangxi 330022,China)

机构地区：[1]江西师范大学生命科学学院,江西南昌330022

出　　处：《江西师范大学学报（自然科学版）》2019年第3期248-252,共5页Journal of Jiangxi Normal University(Natural Science Edition)

基　　金：国家自然科学基金(81660597;81360614);江西省自然科学基金(20132BAB204023)资助项目

摘　　要：为探究不同色光对蛇足石杉离体叶状体生长和石杉碱甲(Hup A)累积的作用,设置了二极管白光(W)、二极管蓝光(B)、二极管红光(R) 3种不同色光处理,以普通荧光灯(CK)为对照,研究不同色光下叶状体生长和Hup A累积的差异及其与IAA、ZT、ABA、GA几种内源激素水平的关系.结果表明:红光处理对蛇足石杉离体叶状体生长繁殖有促进效果,但对Hup A含量累积有极显著的抑制作用;蓝光相较CK提高了叶状体的生长量和Hup A的含量,其叶状体Hup A生产率最高达1 811. 728μg·L^(-1),较CK提高了49. 67%;蓝光处理的叶状体IAA/ZT值、ABA含量、ABA/ZT值、ABA/IAA值在全生育期均高于CK.依据不同类型激素功能,该研究结果说明:蓝光培养的叶状体中存在高水平的生长型激素IAA/ZT,促进其生长,增加了生物量,且其体内成熟型激素ABA的高水平存在,促进了次级代谢产物Hup A累积增加.For exploring the effect of different colored lights on the growth and Huperzine A accumulation of the thallus of Huperzia serrata set up several different colored lights treatment including diode white light (W) diode blue light (B) diode red light (R) are set up to treat. Compared with the common fluorescent lamp(CK) and studied the difference of the growth and HupA accumulation of the thallus under different colored lights and the re lationship between them and endogenous hormone including IAA ZT and ABA GA are studied. The results show that red light is helpful to promote the growth and proliferation of the thallus of Huperzia serrata in vitro but it had an extremely significant inhibitory effect on content of HupA. Compared with CK blue light treatment increase the growth amount and HupA content of the thallus and the HupA productivity is highest upto1811.728μg L^-1 whichishigher49.67% thanCK.ThetotalIAA/ZTvalue theABAcontent ABA/ZTvalue ABA/TAAvalueof the thallus treated with diode blue light are all higher than CK during full period. Depending on the function of dif ferent types of hormones the results of this study indicate that there is high level of growth hormone IAA/ ZT in the thallus treated with blue light which promotes its growth and increased the biomass and the high level of ABA pro motes the accumulation of secondary metabolite HupA.

关 键 词：蛇足石杉 不同色光 生长 石杉碱甲 内源激素 

分 类 号：S567.239[农业科学—中草药栽培]