散养黄羽肉鸡CAPN1、H-FABP基因表达的发育性变化及其肌内脂肪含量的研究  

Developmental Changes of CAPN1 and H-FABP Genes Expression and Their Intramuscular Fat Content in Yellow Feather Broilers Under Free-range

作　　者：朱梦婷[1] 解一凡 王晓路 赵宗胜[1] ZHU Mengting;XIE Yifan;WANG Xiaolu;ZHAO Zongsheng(College of Animal Science and Technology,Shihezi University,Shihezi 832003,China)

机构地区：[1]石河子大学动物科技学院,石河子832003

出　　处：《中国畜牧兽医》2019年第5期1413-1420,共8页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基因项目(31460587)

摘　　要：为探讨在散养条件下黄羽肉鸡CAPN1、H-FABP基因与其生长发育和肌内脂肪(IMF)含量的关系,试验以130羽3周龄体重接近,健康商品代黄羽肉鸡母鸡为试验材料,随机分为笼养组(对照组)和散养组(试验组),采集在不同周龄(3、4、6、8、10、13 w)的胸肌、腿肌和腹脂组织样品共360份,采用实时荧光定量PCR法对不同生长阶段组织中CAPN1和H-FABP基因mRNA表达量进行检测,同时采用索氏抽提法测定胸肌和腿肌的IMF含量。结果表明:黄羽肉鸡的CAPN1、H-FABP基因在胸肌、腿肌和腹脂中均有不同程度的表达。随着周龄的增加,散养肉鸡CAPN1基因表达的整体变化趋势为增加—减少—增加—减少,且3～6 w其在胸肌和腹脂中的表达量均表现为笼养显著高于散养(P<0.05)。H-FABP基因的整体表达趋势随着周龄的增加而降低,在胸肌和腿肌中变化趋势相同,且均表现为散养显著高于笼养(P<0.05),在腹脂中表现为笼养高于散养(P>0.05)。与散养鸡相比,笼养鸡生长速度较快,腹脂率、IMF含量较高。本试验结果可为散养黄羽肉鸡CAPN1和H-FABP基因分子选育提供一定参考。In order to investigate the relationship between CAPN1,H-FABP genes and the growth and intramuscular fat contentcontent (IMF) of Yellow-feathered broilers under the free-range feeding system,130 3-week-old health Yellow-feathered broiler hens with similar weight were selected as test material and randomly divided into two groups with cage rearing (control group) and free-range group (test group).A total of 360 samples of chest muscles,leg muscles and abdominal fat of Yellow-feathered broiler hens were collected,the CAPN 1 and H-FABP genes mRNA expression were analyzed by Real-time quantitative PCR,and the IMF content was determined by the method of Soxhlet extraction.The results showed that the CAPN1 and H-FABP mRNAs of Yellow-feathered broilers were expressed in different degrees in the chest muscles,leg muscles and abdominal fat.With the increase of age,the overall expression trend of CAPN1 in free-range group was increase-decrease-increase-decrease,and the expression level of CAPN1 mRNA in chest and abdominal fat were significantly higher in cage rearing than that in free-range during 3 to 6 w ( P< 0.05).The overall expression trend of H-FABP decreased with the increasing age,and the expression trends in the chest and leg muscles were the same and they were significantly higher in free-range than the cage rearing ( P< 0.05).The expression of H-FABP in abdominal fat was higher in cage rearing group ( P >0.05).Compared to free-range chickens,cage rearing chickens grew faster,and the rate of abdominal fat and IMF content were higher also.The test results could provide some reference for the CAPN 1 and H-FABP genes molecular breeding of Yellow-feathered chickens.

关 键 词：黄羽肉鸡 CAPN1基因 H-FABP基因 肌内脂肪 

分 类 号：S813[农业科学—畜牧学]