MicroRNA-27a靶向PHLPP2促进胃癌细胞增殖和转移的机制探讨  

Mechanism of MicroRNA-27a targeting PHLPP2 promoting proliferation and metastasis of gastric cancer cells

作　　者：范波[1] 施莉[2] FAN Bo;SHI Li(Department of Clinical Laboratory,Jinhua People's Hospital in Zhejiang Province,Jinhua 321000,China;Department of Gastroenterology,Jinhua People's Hospital in Zhejiang Province,Jinhua 321000,China)

机构地区：[1]浙江省金华市人民医院检验科,浙江金华321000 [2]浙江省金华市人民医院消化科,浙江金华321000

出　　处：《中国现代医生》2019年第11期1-4,共4页China Modern Doctor

基　　金：浙江省科技计划项目(2014C33140)

摘　　要：目的研究MicroRNA-27a靶向PHLPP2促进胃癌细胞增殖和转移的机制。方法选取我院2016年1月~2018年1月收治的胃癌患者60例,提取所有患者的胃癌组织和胃癌旁组织,研究MicroRNA-27a靶向PHLPP2促进胃癌细胞增殖和转移的机制。结果在SGC-7901细胞中,miR-27a antagomir与vector-3'UTR-wt共转染后可能会引起报告载体荧光素酶活性的上升,但是在miR-27a antagomir与vector-3'UTR-wt共转染之后,荧光素酶的活性并没有发生较大变化。在AGS细胞中,miR-27a antagomir与vector-3'UTR-wt共转染可能会引起报告载体荧光素酶活性的下降,但是在miR-27a antagomir与vector-3'UTR-wt共转染的报告载体中,荧光素酶的活性并没有发生较为显著的变化。转染miRa antagomir后,SGC-7901细胞中PHLPP2中的mRNA水平和蛋白水平明显上升,转染MicroRNA-27a agomir后,AGS细胞中的PHLPP2的mRNA水平和蛋白水平明显下降(P均<0.05)。结论 miR-327a在胃癌组织和胃癌细胞系中表达上调,MicroRNA-27a能够在胃癌细胞中对PHLPP2的活性产生抑制作用,并且能够促进胃癌细胞的凋亡,对胃癌患者的治疗能够产生一定的积极作用。Objective To explore the mechanism of MicroRNA-27a targeting PHLPP2 promoting proliferation and metastasis of gastric cancer cells. Methods Sixty patients with gastric cancer admitted to our hospital from January 2016 to January 2018 were enrolled. The gastric cancer tissues and paracancerous tissues of all patients were extracted to study the mechanism of MicroRNA-27a targeting PHLPP2 to promote the proliferation and metastasis of gastric cancer cells. Results In SGC-7901 cells, co-transfection of miR-27a antagomir and vector-3'UTR-wt may cause an increase in luciferase activity in the reporter vector, but after co-transfection of miR-27a antagomir and vector-3'UTR-wt, there was no significant change in the activity of luciferase. In AGS cells, co-transfection of miR-27a antagomir and vector-3'UTR-wt may cause a decrease in luciferase activity in the reporter vector, but also no significant change was observed after co-transfection. After transfection of miRa antagomir, the mRNA level and protein level of PHLPP2 in SGC-7901 cells increased significantly. After transfection of MicroRNA-27a agomir, the mRNA and protein levels of PHLPP2 in AGS cells decreased significantly(P<0.05). Conclusion The expression of miR-327a is up-regulated in gastric cancer tissues and gastric cancer cell lines. MicroRNA-27a can inhibit the activity of PHLPP2 in gastric cancer cells and promote the apoptosis of gastric cancer cells, which has positive effects on the treatment of gastric cancer.

关 键 词：MicroRNA-27a 靶向PHLPP2 胃癌细胞 增殖 转移 机制 

分 类 号：R735.2[医药卫生—肿瘤]