非小细胞肺癌血清中IL-2、IL-6、TNF-α和IFN-γ变化与生存率相关性研究  

Correlation between changes of serum IL-2, IL-6, TNF-α and IFN-γ in serum of non-small cell lung cancer and survival rate

作　　者：扶志敏[1] 邝世晏[1] 李炽光 刘俊永 朱云[1] 陈尚志[3] FU Zhimin;KUANG Shiyan;LI Chiguang;LIU Junyong;ZHU Yun;CHEN Shangzhi(Department of Cardiothoracic Surgery,Chenzhou First People's Hospital in Hu'nan Province,Chenzhou,423000,China;Department of Respiratory Medicine,Chenzhou First People's Hospital in Hu'nan Province,Chenzhou,423000,China;Office,Chenzhou First People's Hospital in Hu'nan Province,Chenzhou,423000,China)

机构地区：[1]湖南省郴州市第一人民医院胸心外科,湖南郴州423000 [2]湖南省郴州市第一人民医院呼吸内科,湖南郴州423000 [3]湖南省郴州市第一人民医院办公室,湖南郴州423000

出　　处：《中国现代医生》2019年第11期32-36,共5页China Modern Doctor

基　　金：浙江省郴州市科技局科技计划项目(CZ2014026)

摘　　要：目的明确非小细胞肺癌患者外周血细胞因子IL-2、IL-6、TNF-α和IFN-γ变化及与生存率的相互关系。方法招募2016~2017年53例非小细胞肺癌住院患者和53例健康对照者作为研究对象。收集非小细胞肺癌组入院第7天、30d、90d、180d血清,ELSIA法检测并比较肺癌组和健康组血清中IL-2,IL-6,TNF-α和IFN-γ不同时间段表达差异。通过分析不同时间段非小细胞肺癌组血清中细胞因子与体重相互关系及COX回归生存分析明确影响非小细胞肺癌生存率的细胞因子。结果与健康对照组比较,非小细胞肺癌组血清中细胞因子IL-6和IFN-γ在第7天、30d、90d、180d均出现明显升高(P<0.001,P<0.001,P<0.001,P<0.001和P=0.161,P<0.001,P=0.001,P=0.041);而IL-2,TNF-α水平随住院时间则无明显变化。进一步分析发现非小细胞肺癌第90天和180天血清中IL-6与体重呈负相关(R=-0.368,P=0.045和R=-0.497,P=0.011)。COX回归生存分析发现非小细胞肺癌组IL-6水平与患者生存率存在负相关(P=0.023)。结论非小细胞肺癌会引起血清中IL-6持续升高,持续升高的IL-6可与非小细胞肺癌患者的生存率减少密切相关,血清中IL-6可作为非小细胞肺癌发生发展的生物标记物之一。Objective To determine the changes of peripheral blood cytokines IL-2, IL-6, TNF-α and IFN-γ in patients with non-small cell lung cancer and their relationship with survival rate. Methods A total of 53 non-small cell lung cancer hospitalized patients and 53 healthy controls were enrolled from 2016 to 2017. The sera of the non-small cell lung cancer group were collected on the 7 th, 30 th, 90 th, and 180 th day of the hospital. ELSIA method was used to detect and compare the expression differences in serum IL-2, IL-6, TNF-α and IFN-γ in the lung cancer group and the healthy group over time. The cytokines affecting the survival rate of non-small cell lung cancer were identified by analyzing the relationship between cytokine and body weight in serum and COX regression survival analysis in non-small cell lung cancer group at different time points. Results Compared with those of the healthy control group, the serum cytokines IL-6 and IFN-γ in the non-small cell lung cancer group were significantly increased on the 7 th, 30 th, 90 th, and 180 th days (P<0.001, P<0.001, P<0.001, P<0.001 and P=0.116, P<0.001, P=0.001, P=0.041);while IL-2, TNF-α levels in the non-small cell lung cancer group did not change significantly with hospital stay. Further analysis found that serum IL-6 was negatively correlated with body weight on 90th days and 180th days of non-small cell lung cancer (R=-0.368, P=0.045 and R=-0.497, P=0.011). COX regression survival analysis found that IL-6 levels in non-small cell lung cancer group were negatively correlated with patient survival(P=0.023). Conclusion Non-small cell lung cancer can cause continuous increase of serum IL-6. The continuous increase of IL-6 is closely related to the decrease of survival rate in patients with non-small cell lung cancer. IL-6 in serum can be used as one of the biomarkers for the development of non-small cell lung cancer.

关 键 词：非小细胞肺癌 血清 IL-6 生存率 

分 类 号：R730.4[医药卫生—肿瘤]