miR-146a多态性与非小细胞肺癌易感性的研究  

Association between polymorphisms in the miR-146a and susceptibility to non-small cell lung cancer

作　　者：应伟[1] 王兴远[1] 江波[1] 周伟[1] YING Wei;WANG Xingyuan;JIANG Bo;ZHOU Wei(Department of Oncology,People’s Hospital ofGuang’an,Guang’an 638000,Sichuan,China)

机构地区：[1]广安市人民医院肿瘤科

出　　处：《癌变．畸变．突变》2019年第3期222-226,共5页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的:研究汉族人群miR-146a rs2910164 G/C基因多态性与非小细胞肺癌易感性的关系。方法:通过病例-对照研究,应用PCR-限制性片段长度多态性(PCR-RFLP)检测技术对198例非小细胞肺癌患者与218例对照组人群进行rs2910164基因型的检测,并随机抽取10%的样本进行DNA测序,进行遗传平衡检测。进一步采用Logistic回归分析该位点与非小细胞肺癌的相关性。结果:rs2910164 被酶切成GG、GC、CC 基因型,GG、GC、CC 分型在对照组分别为103 例(47.25%)、85 例(38.99%)、30 例(13.76%);在病例组分别为31例(15.66%)、99例(50.00%)、68例(34.34%)。随机抽取10%的样本进行DNA测序,其结果与PCRRFLP分型结果一致,基因分型频率满足Hardy-Weinberg遗传平衡(P>0.05)。与对照组相比,Logistic回归分析发现携带C等位基因的基因型可明显增加非小细胞肺癌的发病风险[显性模型OR=5.04,95%CI 为(4.72,5.39),P<0.01;隐性模型OR=2.75,95%CI 为(2.57,2.94),P<0.01];而rs2910164 基因多态性与非小细胞肺癌的临床病理特征(分级、分期、转移)之间无明显相关关系(P>0.05),rs2910164基因多态性与吸烟之间无交互作用(P>0.05)。结论:携带miR-146a rs2910164 C等位基因的基因型可能与非小细胞肺癌的遗传易感性相关。OBJECTIVE:To study the relationship between miR-146a rs2910164 G/C gene polymorphism and non-small cell lung cancer in the Han population. METHODS:rs2910164 genotypes in 198 patients with non- small cell lung cancers and 218 control subjects were detected by PCR- restriction fragment length polymorphism (PCR- RFLP). Logistic regression analysis was used. RESULTS: Compared with the control group,logistic regression analyses show that the genotype carrying the C allele was significantly associated with increased risk of non- small cell lung cancer [dominant model OR=5.04, 95% CI (4.72, 5.39), P<0.01;recessive model OR=2.75, 95% CI was (2.57, 2.94), P<0.01]. Further analyses show that there was no statistical relationship between the rs2910164 gene polymorphism and clinical features (gradation, stage, metastasis)(P>0.05) and there was no interaction between the rs2910164 gene polymorphism and smoking (P> 0.05). CONCLUSION:Our results show that the genotype carrying the miR-146a rs2910164 C allele may be associated with genetic susceptibility to non-small cell lung cancer.

关 键 词：单核苷酸多态性 遗传易感性 非小细胞肺癌 MIR-146A 

分 类 号：R734.2[医药卫生—肿瘤]