低热环境影响结直肠癌细胞增殖凋亡的机制研究  

Mechanism of proliferation and apoptosis of colorectal cancer cells affected by low-grade fever environment

作　　者：杜晓翔[1] 叶蕾 DU Xiaoxiang;YE lei(Department of Pharmacy,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325000,China)

机构地区：[1]温州医科大学附属第一医院药剂科,浙江温州325000

出　　处：《全科医学临床与教育》2019年第5期405-408,共4页Clinical Education of General Practice

摘　　要：目的探讨低热环境对结直肠癌细胞增殖凋亡的影响。方法分别以37℃,5%CO2恒温培养和39℃,5%CO2恒温培养的结直肠癌细胞株Caco-2为低热组和对照组。采用实时无标记细胞分析技术(RTCA)观察两组细胞增殖抑制情况;采用流式细胞术检测活性氧水平与线粒体膜电位;采用实时定量聚合酶链式反应(QPCR)检测增殖凋亡相关基因半胱天冬氨酸蛋白水解酶-3(Caspase-3)、B淋巴细胞瘤-2(Bcl-2)及热休克蛋白70(HSP70)等的表达情况。结果 RTCA结果显示:对照组于铺板后的48 h左右进入平台期,低热组于铺板后的24 h左右进入平台期;流式细胞术结果显示:低热组的活性氧与线粒体膜电位与对照组差异不大;QPCR结果显示:相较于对照组,低热组Caspase-3表达下调,Bcl-2表达上调,同时HSP70的表达明显上调,差异均有统计学意义(t分别=17.29、-33.38、-539.54,P均<0.05)。结论低热环境未对细胞产生明显的损伤,反而使得HSP70表达明显提高,并可促进结直肠癌细胞的增殖同时抑制细胞凋亡。Objective To investigate the effect of low-grade fever on the proliferation and apoptosis of colorectal cancer cells. Methods Colorectal cancer cell lines Caco-2 were cultured at 37 ℃,5% CO2 and 39 ℃,5% CO2 constant temperature incubator as low-grade fever group and control group,respectively.RTCA real-time unlabeled cell assay was used to observe the cell growth and the inhibition of proliferation.Reactive oxygen species(ROS)level and mitochondrial membrane potential were detected by flow cytometry.The expressions of Caspase-3,B-cell lymphoma-2(Bcl-2) and Heat shock protein70(HSP70)were detected by QPCR. Results RTCA results showed that the low-grade fever group entered the plateau stage earlier than the control group.Flow cytometry showed that the ROS level and mitochondrial membrane potential of the low-grade fever group were not significantly different from those of the control group.QPCR results showed that Caspase-3 expression was down-regulated,Bcl-2 expression was up-regulated,and HSP70 expression was significantly up-regulated in the low-grade fever group when compared with the control group(t=17.29,-33.38,-539.54,P<0.05). Conclusion Low-grade fever environment did not cause obvious damage to cells,but increased the expression of HSP70 significantly,and promoted the proliferation of colorectal cancer cells and inhibited apoptosis.

关 键 词：结直肠癌 低热 增殖 凋亡 半胱天冬氨酸蛋白酶-3 B淋巴细胞瘤-2 热休克蛋白70 

分 类 号：R735.3[医药卫生—肿瘤]