不同的洋地黄类药物对甲状腺乳头状癌细胞自噬的影响  

Digitalis-like Compounds Facilitating Autophagy in Papillary Thyroid Carcinoma

作　　者：马金玲 王丹[2] 段滨红[2] 邹宁 王亦薇[2] 杜馥曼[2] 刘余[2] 赵凌斐[2] 仲恒[2] 卞丙凤 MA Jin-ling;WANG Dan;DUAN Bin-hong(School of Post Graduate,Jiamusi University,Jiamusi,Hei longjiang,154007,China)

机构地区：[1]佳木斯大学研究生部,黑龙江佳木斯154007 [2]黑龙江省医院内分泌科,哈尔滨150036 [3]哈尔滨医科大学中国疾病预防控制中心地方病控制中心,哈尔滨150081

出　　处：《黑龙江医学》2019年第5期442-443,共2页Heilongjiang Medical Journal

基　　金：黑龙江省卫建委科研课题资助项目(2018-091);黑龙江省自然科学基金面上项目(H2015085)

摘　　要：目的探讨不同的洋地黄类药物对甲状腺乳头状癌细胞株BC-PAP的自噬调控作用。方法将甲状腺乳头状癌细胞株BC-PAP分为空白对照组、洋地黄毒苷组、地高辛组,应用CCK-8方法测定细胞活性,并观察细胞的生长情况,电镜观察细胞内自噬小体的形成。结果洋地黄毒苷、地高辛均能够呈剂量依赖性地抑制BC-PAP细胞的生长。洋地黄毒苷的IC50浓度为0.25μmol/L,而地高辛的IC50浓度为5μmol/L。结论洋地黄毒苷、地高辛可增强甲状腺癌细胞自噬,洋地黄毒苷诱自噬的作用更强。Objective To investigate digitalis-like compounds facilitating autophagy in papillary thyroid carcinoma. Methods BC-PAP cells were divided into three groups:control,digitoxin and digoxin group. The half-inhibition rate of the cells was calculated by cck-8 experiment. The grouth condition of cells was abserved cells of each group were cultured for 12 hand observed under transmission electron microscope. The formation of autophagosomes in cells was observed under electron microscope. Results Under electron microscope, the formation of autophagosomes in digitoxin group and digoxin group were significantly higher than those in the blank control group. IC50 of digitoxin was 0.25 μmol/L. IC50 of digoxin was 5 μmol/L. Conclusion Digitalis-like compounds can increase autophagy in papillary thyroid carcinoma. Digitoxin was more powerful than digoxin.

关 键 词：甲状腺乳头状癌 自噬 洋地黄毒苷 地高辛 

分 类 号：R736.1[医药卫生—肿瘤]