p15^(PAF)蛋白对食管癌细胞糖酵解水平的影响  

The influence of p15^(PAF) protein on glycolysis of esophageal cancer cells in vitro

作　　者：程遥[1] 刁冬梅[2] 李少民[1] 周斌[1] 乔哲[1] 宋永春[2] 李康[2] 党诚学[2] Cheng Yao;Diao Dongmei;Li Shaomin;Zhou Bin;Qiao Zhe;Song Yongchun;Li Kang;Dang Chengxue(Department of Thoracic Surgery,the Second Affiliated Hospital,Xi'an Jiaotong University College of Medicine,Shaanxi Xi'an 710004,China;Department of Surgical Oncology,the First Affiliated Hospital,Xi'an Jiaotong University College of Medicine,Shaanxi Xi'an 710061,China)

机构地区：[1]西安交通大学第二附属医院胸外科,陕西西安710004 [2]西安交通大学第一附属医院肿瘤外科,陕西西安710061

出　　处：《现代肿瘤医学》2019年第11期1849-1852,共4页Journal of Modern Oncology

基　　金：国家自然科学基金(编号:81501826);陕西省社会发展科技攻关项目(编号:2016SF-198;2016SF-184)

摘　　要：目的:探讨p15^(PAF)蛋白对食管癌细胞糖酵解水平及相关生物学效应的影响。方法:使用细胞活性检测及Western Blot等方法对细胞耗糖量、2-脱氧葡萄糖(2-DG)的敏感性及自噬水平等生物学效应进行检测。结果:上调p15^(PAF)蛋白的食管癌Eca-109及TE-1细胞较对照组有更高的增殖能力和葡萄糖消耗,但对2-DG造成的细胞增殖抑制更为敏感,下调p15^(PAF)蛋白的细胞细胞增殖能力下降,该现象与Akt信号通路有关。另外,p15^(PAF)蛋白还可以通过Akt信号通路下调食管癌细胞的基础自噬水平。结论:p15^(PAF)蛋白可以通过PI3K-Akt信号通路影响食管癌细胞的糖酵解及自噬水平。Objective:To discover the effect of p15^PAF protein on glycolysis of esophageal cancer cells.Methods:We used MTT test to detect cell glucose comsumption,and sensitivity of 2-dexyo-glucoseand we performed Western Blot of LC-ⅢB to analyze the level of autophagy,HK-2 and GLUT-1 to assess the glycolysis.Results:Elevated p15^PAF protein increased proliferation ability and glucose comsumption,while significantly inhibited when incubating with 2-DG.Down regulation of p15^PAF caused the opposite result,and this phenomenon related with Akt signaling.On the other hand,p15^PAF protein decreased basic autophagy ability via Akt signaling with the mechanism needing studied.Conclusion:p15^PAF protein may increase glycolysis ability and decrease basic autophagy by activating PI3 K-Akt signaling.

关 键 词：食管癌 p15^PAF蛋白 糖酵解 

分 类 号：R735.1[医药卫生—肿瘤]