CK10和CK17在早期宫颈癌组织中的表达及其预测预后的价值  

The expression and prognostic value of CK10 and CK17 in early cervical cancer tissue

作　　者：赵亚琼 任琛琛[1] 杨立[1] 刘灵[1] 张小安[2] 曾宪旭[3] 党秋红[3] Zhao Yaqiong;Ren Chenchen;Yang Li;Liu Ling;Zhang Xiaoan;Zeng Xianxu;Dang Qiuhong(Department of Gynaecology,Third Affiliated Hospital of Zhengzhou University,Henan Zhengzhou 450052,China;Department of Radiology,Third Affiliated Hospital of Zhengzhou University,Henan Zhengzhou 450052,China;Department of Pathology,Third Affiliated Hospital of Zhengzhou University,Henan Zhengzhou 450052,China)

机构地区：[1]郑州大学第三附属医院妇科,河南郑州450052 [2]郑州大学第三附属医院放射科,河南郑州450052 [3]郑州大学第三附属医院病理科,河南郑州450052

出　　处：《现代肿瘤医学》2019年第11期1961-1967,共7页Journal of Modern Oncology

基　　金：河南省教育厅重大科技专项(编号:161100311100);河南省教育厅项目(编号:19A320052);河南省医学科技攻关计划省部共建项目(编号:201601010)

摘　　要：目的:CK10和CK17在早期宫颈癌组织中的表达及其预测预后价值的意义。方法:采用标准免疫组化SP法检测CK10和CK17在60例正常宫颈组织、60例宫颈上皮内瘤变和120例宫颈癌组织中的表达情况。结果:CK10的阳性表达定位于细胞质,在正常宫颈组织、CIN、宫颈癌患者中阳性表达率分别为80.00%、50.00%和21.67%,三组相比差异有显著统计学意义(P<0.001)。CK17的阳性表达定位于细胞质,三组阳性表达率分别为0%、46. 67%和80. 00%,三组相比差异显著(P <0. 001)。χ2检验分析宫颈癌组织中CK17的表达与患者FIGO分期、是否有淋巴结转移、病理分型、组织分化无关(P> 0. 05); CK10的表达与患者FIGO分期、是否有淋巴结转移、病理分型无关,与组织分化程度有关(P=0. 021)。Kaplan-Meier生存分析显示:早期宫颈癌手术治疗患者的1年、3年、5年生存率分别为:100%、96. 7%、93. 3%; CK10阴性组和阳性组5年生存率分别为91. 5%和100%; CK17阴性组和阳性组5年生存率分别为100%和91. 7%,差异均无统计学意义(P> 0. 05)。单因素分析显示CK10及CK17的表达和预后无关(P> 0. 05),年龄、是否淋巴结转移、FIGO分期、组织分化、病理分型均与预后相关(P <0. 05)。根据CK17和CK10的表达情况将患者分为四组,CK17阳性CK10阴性组患者1年、3年、5年生存率分别为:100%、94. 9%、89. 7%,余CK17阳性CK10阳性组、CK17阴性CK10阳性组、CK17阴性CK10阴性组5年生存率分别为:100%、100%、100%,差异无统计学意义(P=0. 211)。结论:CK10的表达水平与宫颈病变严重程度呈负相关,CK17的表达水平与宫颈病变严重程度呈正相关,两者可能参与了宫颈癌的发生发展,但CK17和CK10在预测宫颈癌患者预后方面的价值不大,而年龄、是否淋巴结转移、FIGO分期、组织分化、病理分型是影响患者预后的重要因素。Objective:To study the expression and prognostic value of CK10 and CK17 in patients undergoing early cervical cancer surgery.Methods:Standard immunohistochemical SP method was used to detect the expression of CK10 and CK17 in 60 normal cervical tissues, 60 cervical intraepithelial neoplasia and 120 cervical cancer tissues. Results:The positive expression of CK10 was located in cytoplasm,and the positive expression rates were 80.00%,50.00%,and 21.67% in normal cervical tissues,CIN,and cervical cancer patients,respectively,showing a significant difference among the three groups(P<0.001).The positive expression of CK17 was located in cytoplasm.The positive expression rates of the three groups were 0%,46.67% and 80.00%,respectively,with significant differences(P<0.001).The χ^2 test analyzed that the expression of CK17 in cervical cancer tissues was independent of FIGO stage,lymph node metastasis,pathological classification and tissue differentiation(P>0.05).The expression of CK10 was independent of the patient’s FIGO stage,lymph node metastasis or pathological typing,and was related to the degree of tissue differentiation(P=0.021).Kaplan-Meier survival analysis showed that the 1-year,3-year and 5-year survival rates of patients with early cervical cancer treated by surgery were 100%,96.7% and 93.3%,respectively.The 5-year survival rates in the CK10 negative group and the positive group were 91.5% and 100%,respectively(P>0.05). The 5-year survival rate of the CK17 negative group and the positive group was 100% and 91.7%,respectively(P>0.05).Univariate analysis showed that the expression of CK10 and CK17 was not correlated with prognosis(P>0.05),and age,lymph node metastasis,FIGO stage,tissue differentiation,and pathological classification were significantly correlated with prognosis(P<0.05).According to the expression of CK17 and CK10,we divided the patients into four groups.The 1-year,3-year and 5-year survival rate of CK17 positive CK10 negative group was:100%,94.9%,89.7%.The 5-year survival rate of CK17 positive

关 键 词：CK10 CK17 宫颈癌 宫颈癌预后 

分 类 号：R737.33[医药卫生—肿瘤]