绵羊GnIH和VIP基因在不同繁殖状态下的表达变化  

Expression Pattern of GnIH and VIP Genes in Different Reproductive States of Sheep

作　　者：李春艳 刘秋月[1] 胡文萍[1] 王翔宇[1] 马琳[1] 张效生[3] 张金龙[3] 狄冉[1] 储明星[1] LI Chunyan;LIU Qiuyue;HU Wenping;WANG Xiangyu;MA Lin;ZHANG Xiaosheng;ZHANG Jinlong;DI Ran;CHU Mingxing(Key Laboratory of Animal Genetics,Breeding and Reproduction of Ministry of Agriculture,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China;School of Life Sciences,Anhui Agricultural University,Hefei 230036,China;Tianjin Institute of Animal Sciences,Tianjin 300381,China)

机构地区：[1]中国农业科学院北京畜牧兽医研究所农业部动物遗传育种与繁殖重点实验室,北京100193 [2]安徽农业大学生命科学学院,合肥230036 [3]天津市畜牧兽医研究所,天津300381

出　　处：《畜牧兽医学报》2019年第5期930-938,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31572371;31472078);国家肉羊产业技术体系专项(CARS-38);中央级公益性科研院所基本科研业务费专项(Y2017JC24);中国农业科学院科技创新工程(ASTIP-IAS13);农业科研杰出人才及其创新团队项目(农办人[2015]62号);国家万人计划科技创新领军人才项目(W02020274);天津市农业科技成果转化与推广项目(201704020)

摘　　要：旨在进一步阐明GnIH和VIP基因在绵羊季节性发情和繁殖调控中的作用。本研究选取短光照(模拟繁殖季节)和长光照(模拟休情期)下成年苏尼特母羊各3只及卵泡期和黄体期的小尾寒羊母羊各3只,同时选取短光照第21天和长光照第3、15、21、42、49天共6个不同光照时间点的成年苏尼特母羊各3只,屠宰后采集其性腺轴组织(下丘脑、垂体、松果体、卵巢、输卵管、子宫体),利用qPCR技术分析GnIH和VIP基因mRNA表达规律。结果表明,GnIH和VIP基因在2个绵羊品种上述各组织中均表达,且在下丘脑中的表达量较高;苏尼特羊下丘脑中2个基因在长光照条件下的表达量均极显著高于短光照条件(P<0.01);在小尾寒羊下丘脑组织中,黄体期VIP基因的表达量显著高于卵泡期(P<0.05),黄体期GnIH基因的表达量稍高于卵泡期,但差异不显著(P>0.05)。由短光照转至长光照时,苏尼特羊下丘脑中GnIH和VIP基因表达上升,至长光照第3天时,2个基因表达均达到峰值,之后呈下降趋势。本研究揭示了GnIH和VIP基因在季节性发情和常年发情成年绵羊性腺轴各组织中的定量表达特征,不同光照条件和不同繁殖时期绵羊下丘脑中这2个基因的表达变化进一步提示了GnIH和VIP基因参与绵羊季节性发情调控和发情时期转换;在短光照转至长光照过程中,GnIH和VIP基因主要在长光照前3 d发挥关键作用。The aim of this study was to further elucidate the roles of GnIH and VIP genes in sheep seasonal estrus and reproductive regulation.Adult Sunite sheep (SNT) under short photoperiod and long photoperiod and Small Tail Han sheep (STH) at follicular stage and luteal stage were selected,meanwhile SNT at 21th day of short photoperiod and 3rd,15th,21th,42th,49th days of long photoperiod were selected,respectively.Gonadal axis tissue samples including hypothalamus,pituitary,pineal body,ovary,oviduct,uterine body were collected in 3 sheep for each reproductive stage.The qPCR method was used to analyze the expression characteristics of GnIH and VIP genes.The results showed that GnIH and VIP genes were expressed in the above 6 tissues,and had a high expression level in hypothalamus tissue of two breeds (SNT and STH).Expression level of two genes under long photoperiod (LP) was extremely significantly higher than that under short photoperiod (SP) in hypothalamus of SNT ( P <0.01).Expression level of VIP gene was significantly higher at luteal stage than that at follicular stage ( P <0.05),and expression level of GnIH gene was slightly higher at luteal stage than that at follicular stage but the difference was not significant ( P >0.05) in hypothalamus of STH.When SP turned into LP,expression levels of two genes in hypothalamus of SNT rose and showed a transient peak at LP 3rd day,then the expression decreased.Quantitative expression characteristics of GnIH and VIP genes in gonadal axis tissues of adult sheep were revealed in this study.Additionally,expression pattern of the 2 genes in hypothalamus under different photoperiods and different reproduction stages further implied that GnIH and VIP genes was involved in the regulation of sheep seasonal estrus and transition of estrous stages.When SP turns into LP,the 2 genes play pivotal role in the first 3 days under LP.

关 键 词：绵羊 GNIH VIP 繁殖时期 表达模式 

分 类 号：S826.2[农业科学—畜牧学]