一株高效石油降解菌的筛选及降解性能研究  

Screening and biodegradability of a highly efficient petroleum-degrading becterial strain

作　　者：邢清朝[1,2] 习彦花[1] 单胜道 程辉彩[1] 王宏伟[2] 张丽萍[1] XING Qingchao;XI Yanhua;SHAN Shengdao;CHENG Huicai;WANG Hongwei;ZHANG Liping(Institute of Biology,Heibei Academy of Sciences,Shijiazhuang 050081,Hebei,China;College of Life Sciences,Hebei University,Baoding 071000,Hebei,China;School of Environmental and Natural Resources,Zhejiang University of Science and Technology,Hangzhou 310023,Zhejiang,China)

机构地区：[1]河北省科学院生物研究所,石家庄050081 [2]河北大学生命科学学院,河北保定071000 [3]浙江科技学院环境与资源学院,杭州310023

出　　处：《浙江科技学院学报》2019年第3期224-230,共7页Journal of Zhejiang University of Science and Technology

基　　金：河北省高层次人才资助项目(E2015100006);河北省科技计划项目(18393814D);河北省科学院科技项目(18301)

摘　　要：针对石油污染问题,选育高效石油降解菌,为石油污染生物修复提供菌种资源和技术支持。通过连续富集传代培养,从石油污染土壤样品中分离出高效石油降解菌XS-2。经过形态学、生理生化以及16S rRNA序列分析,鉴定XS-2为赤红球菌(Rhodococcus ruber)。该菌的最佳培养条件是培养温度30℃、初始培养pH值7.0、石油质量浓度5 g/L,7 d降解率可达65%。经气相色谱(GC)分析,该菌可有效降解碳14、15、16、17的正构烷烃。因此,赤红球菌XS-2在开发研制石油污染生物修复菌剂方面有较好的应用前景。In response to the oil pollution problem, a highly efficient petroleum-degrading bacterial strain was bred to provide species resources and technical support for bioremediation of oil pollution. Through continuous enrichment subculture, XS -2, the highly efficient petroleum-degrading bacteria was isolated from samples of soil contaminated by petroleum. The strain was identified as Rhodococcus ruber through morphological, physiological and biochemical and 16S rRNA sequence analysis. Its culture conditions were optimized by culture temperature of 30 ℃, initial pH value of 7.0 and petroleum mass concentration of 5 g/L, as a result of which the degradation rate in 7 days could reach up to 65%. Through GC analysis, the strain could effectively degrade n-alkanes with C 14, 15, 16 and 17. Therefore, Rhodococcus ruber XS -2, has sound prospects for application in the aspect of developing bioremediating inoculant of oil pollution.

关 键 词：石油 生物降解 菌种鉴定 降解率 

分 类 号：X172[环境科学与工程—环境科学]