利用生物信息学方法解析自噬相关基因在人体红细胞终末分化各阶段的动态表达  

Dissecting the Dynamic Expression of Autophagy Related Genes at Each Stage of Human Terminal Erythriod Differentiation Through Bioinformatic Methods

作　　者：徐长禄 赵艳红 马艺戈 王冰蕊 王鼎 郭青 佟静媛 高洁 李亚朴 刘金花 石莉红 XU Changlu;ZHAO Yanhong;MA Yige;WANG Bingrui;WANG Ding;GUO Qing;TONG Jingyuan;GAO Jie;LI Yapu;LIU Jinhua;SHI Lihong(State Key Laboratory of Experimental Hematology,Institute of Hematology and Blood Diseases Hospital,Chinese Academy of Medical Science&Peking Union Medical College,Tianjin 300020,China)

机构地区：[1]中国医学科学院&北京协和医学院血液病医院(血液学研究所)实验血液学国家重点实验室,天津300020

出　　处：《生物技术进展》2019年第3期271-276,共6页Current Biotechnology

基　　金：国家自然科学基金项目(81870089);国家重点研发计划项目(2016YFA0102300;2017YFA0103100);中央高校基本科研业务费专项资金(3332018157);中国医学科学院医学与健康科技创新工程项目(2016-I2M-1-018;2016-I2M-3-002;2017-I2M-1-015)资助

摘　　要：为了探究自噬相关基因在人体红细胞终末分化各阶段的表达概况,利用生物信息学方法对人体终末分化各阶段红细胞转录组测序(RNA sequencing,RNA-Seq)数据中自噬相关基因的表达进行提取并分析。结果表明,自噬相关基因在红细胞终末分化各阶段呈现不同的表达模式;在红细胞终末分化的早期,超过50%的自噬相关基因呈现高表达状态;原始红细胞及早幼红细胞中高表达的自噬相关基因富集的生物学过程主要包括细胞自噬及其调节、蛋白质的磷酸化及囊泡的运输,而线粒体自噬相关基因主要在中幼红细胞及晚幼红细胞阶段高表达。研究发现,自噬相关基因在人体红细胞终末分化各阶段的表达呈现动态变化;不同阶段的红细胞中高表达的自噬相关基因及其富集的生物学过程均不同。In order to analyze the dynamic expression of autophagy related genes in human terminal erythroid differentiation, based on their RNA-Seq data, the expression of autophagy related genes in human terminal erythroid differentiation were extracted and analyzed by using bioinformatics methods. Results revealed that the expression patterns of autophagy related genes in different stages of terminal erythroid differentiation were different. In the early stage of terminal erythroid differentiation, more than 50% of the autophagy related genes were highly expressed, and the biological processes which autophagy related genes with high expression level in proerythroblast and basophilic erythroblast enriched included autophagy and its regulation, proteins phosphorylation and transport of vesicles. Besides, mitophagy related genes were highly expressed in polychromatic erythroblast and orthochromatic erythroblast. The research indicated that the expression of autophagy related genes in human terminal erythroid differentiation showed dynamic changes. In addition, autophagy related genes with high expression level in different stages were different, as well as the biological processes they enriched.

关 键 词：自噬相关基因 红细胞终末分化 RNA-SEQ 红细胞成熟 生物信息学分析 

分 类 号：Q811.4[生物学—生物工程] R329.2[医药卫生—人体解剖和组织胚胎学]