机构地区:[1]河南省焦作市人民医院乳甲外科,454300 [2]河南省焦作市人民医院肾内科,454300 [3]郑州大学第一附属医院乳腺外科,450052
出 处:《中华实验外科杂志》2019年第5期812-815,共4页Chinese Journal of Experimental Surgery
摘 要:目的观察人参皂苷Rg3抑制乳腺癌大鼠肿瘤组织中血管生长及对血管内皮生长因子(VEGF)和基质金属蛋白酶(MMP)-9表达的影响。方法复制乳腺癌大鼠模型,分为模型组、低剂量组和高剂量组,每组20只,另取健康20只大鼠为空白对照组。空白对照组和模型组大鼠灌胃0.5%羧甲基纤维素钠溶液,低剂量组和高剂量组均灌胃含有人参皂苷Rg3的0.5%羧甲基纤维素钠溶液。检测各组大鼠瘤体的体积、质量、抑瘤率、微血管密度(MVD)、VEGF和MMP-9蛋白表达水平以及血清中VEGF和MMP-9水平。应用SPSS 19.0统计软件进行分析。结果模型组、低剂量组和高剂量组肿瘤体积分别为(4.83±0.66)、(3.48±0.51)、(1.58±0.23)cm3,肿瘤质量分别为(5.17±0.59)、(4.00±0.42)、(2.09±0.26)g,抑瘤率分别为0.00%、22.43%、53.81%,低剂量组和高剂量组的肿瘤体积和肿瘤质量均显著小于模型组(t=7.238、20.795、7.225、21.364,P<0.05),高剂量组的肿瘤体积和肿瘤质量均显著小于低剂量组(t=15.188、17.292,P<0.05),高剂量组的抑瘤率显著高于低剂量组(χ2=12.324,P<0.05);空白对照组、模型组、低剂量组和高剂量组大鼠肿瘤或乳腺组织中的MVD分别为(7.53±1.53)、(23.52±4.21)、(17.32±3.52)、(12.20±2.19)个,血清中VEGF水平分别为(218.42±46.37)、(435.67±83.21)、(350.13±45.26)、(278.58±30.56)mg/L,血清中MMP-9水平分别为(230.52±32.56)、(460.22±60.36)、(330.73±51.57)、(255.72±41.73)mg/L,组织中VEGF蛋白表达水平分别为(0.23±0.09)、(0.58±0.14)、(0.46±0.12)、(0.25±0.09),组织中MMP-9蛋白表达水平分别为(0.14±0.11)、(0.82±0.22)、(0.63±0.19)、(0.46±0.20),模型组大鼠肿瘤组织中MVD、VEGF和MMP-9均显著高于空白对照组(t=15.964、9.405、12.364,P<0.05),低剂量组和高剂量组大鼠肿瘤组织中MVD、VEGF和MMP-9均显著小于模型组(t=5.053、10.668、2.910、8.867、2.923、5.415,P<0.05),高剂量组大鼠肿瘤组织中MVD、VEGF和MMP-9显著小于Objective To explore the inhibitory effect of ginsenoside Rg3 on vascular growth and expression of vascular endothelial growth factor(VEGF)and matrix metalloproteinase(MMP)-9 in breast cancer rats.Methods The rat model of breast cancer was replicated.The successful rats were divided into model group,low dose group and high dose group according to the random number table method,20 rats in each group,and 20 healthy rats were taken as blank control group.Rats in the blank control group and model group were given 0.5%sodium carboxymethyl cellulose solution by gavage,while those in the low-dose group and the high-dose group were given 0.5%sodium carboxymethyl cellulose solution containing ginsenoside Rg3.The volume,mass,tumor inhibition rate,microvessel density(MVD),expression of VEGF and MMP-9 were measured,and the serum levels of VEGF and MMP-9 were measured.Statisticalanalysis of data using the statistical product and service solutions 19.0 software.Results The tumor volume of model group,low dose group and high dose group were(4.83±0.66),(3.48±0.51),(1.58±0.23)cm3,and the mass of tumors were(5.17±0.59),(4.00±0.42),(2.09±0.26)g,respectively.The inhibition rates were 0.00%,22.43%,53.81%,respectively.The volume and mass of tumors in the low dose group and the high dose group were significantly smaller than those in the model group(t=7.238,20.795,7.225,21.364,P<0.05),the volume and mass of tumors in the high dose group were significantly smaller than those in the low dose group(t=15.188,17.292,P<0.05),and the inhibition rate in the high dose group was significantly higher than that in the low dose group(X^2=12.324,P<0.05).The MVDs in tumors and breast tissues of rats in blank control group,model group,low dose group and high dose group were(7.53±1.53),(23.52±4.21),(17.32±3.52)and(12.20±2.19),respectively.The serum levels of VEGF were(218.42±46.37),(435.67±83.21),(350.13±45.26),(278.58±30.56)mg/L,and the serum levels of MMP-9 were(230.52±32.56),(460.22±60.36),(330.73±51.57),(255.72±41.73)mg/L.The expr
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