过表达线粒体融合蛋白2对膜相关磷脂酰肌醇转移蛋白3阳性的肝癌细胞株SMMC-7721侵袭和迁移的影响  

Effect on invasion and migration of migration of mitofusion-2 overexpression to phosphatidylinositol transfer membrane-associated protein 3-positive hepatoma cell Line SMMC-7721

作　　者：李文滨[1] 彭耀荣[1] 刘纯霞[2] 周振宇[3] 张贺云[3] 万云乐[4] 林泽宇 Li Wenbin;Peng Yaorong;Liu Chunxia;Zhou Zhenyu;Zhang Heyun;Wan Yunle;Lin Zeyu(Department of Biliopancreatic Surgery,Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou 510120,China;Department of Ultrasonography,Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou 510120,China;Department of Hepatobiliary Surgery,Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou 510120,China;Department of Hepatobiliary Surgery,the Sixth Affiliated Hospital of Sun Yat-sen University,Guangzhou 510655,China)

机构地区：[1]中山大学孙逸仙纪念医院胆胰外科,广州510120 [2]中山大学孙逸仙纪念医院超声科,广州510120 [3]中山大学孙逸仙纪念医院肝胆外科,广州510120 [4]中山大学附属第六医院肝胆外科,广州510655

出　　处：《中华实验外科杂志》2019年第5期830-833,共4页Chinese Journal of Experimental Surgery

摘　　要：目的观察过表达线粒体融合蛋白2(Mfn2)对膜相关磷脂酰肌醇转移蛋白3(PITPNM3)表达阳性肝癌细胞株侵袭和迁移的作用。方法以PITPNM3表达阳性乳腺癌细胞株McF-7作为阳性对照组,筛选PITPNM3表达阳性的SMMC-7721肝癌细胞株用于实验,使用CC型趋化因子配体18(CCL18),以20μg/L的CCL18浓度建立PITPNM3表达阳性肝癌细胞侵袭和迁移模型;于PITPNM3表达阳性肝癌细胞转染含Mfn2腺病毒载体36 h后,检测PITPNM3表达变化以及其对肝癌细胞侵袭和迁移的影响。应用SPSS 19.0统计软件进行分析。结果肝癌细胞株SMMC-7721中PITPNM3的mRNA呈高表达,相对表达量为3.4;HepG2中PITPNM3相对表达量为1.6,McF-7中PITPNM3相对表达量为4.5,SMMC-7721与HepG2比较,差异有统计学意义(t=3.653,P<0.05);SMMC-7721与McF-7比较,差异无统计学意义(t=0.915,P>0.05);20μg/L的CCL18可有效下调肝癌细胞株SMMC-7721中E-Cadherin表达,上调Snail和Vimentin的表达,促进SMMC-7721侵袭和迁移;Mfn-2可导致肝癌细胞株SMMC-7721的PITPNM3蛋白表达显著下降,侵袭和迁移能力受到抑制,但并未下调PITPNM3的mRNA量,实验组PITPNM3的mRNA相对表达量为0.903,阳性对照组PITPNM3的mRNA相对表达量为1.128,组间比较差异无统计学意义(t=0.577,P>0.05)。结论Mfn2可有效下调PITPNM3表达,抑制肝癌细胞株SMMC-7721侵袭和迁移的能力。Objective To investigate the effect on invasion and migration of mitofusion-2(Mfn-2)overexpression to phosphatidylinositol transfer membrane-associated protein 3(PITPNM3)-positive hepatoma cell Line SMMC-7721.Methods PITPNM3 positive liver cancer cell line was selected for experiment while PITPNM3 positive breast cancer cell line McF-7 was used as a positive control group.20μg/L CC-chemokine ligand 18(CCL18)was used to establish a PITPNM3 positive hepatoma cell model with invasion and migration.PITPNM3 positive hepatoma cell were transfected Mfn-2 with an adenoviral vector after 36 h to detect the change of PITPNM3 expression and its effect on invasion and migration of hepatoma cells.Results Compared to other groups,PITPNM3 was highly expressing in hepatoma cell line SMMC-7721,the relative expression level of PITPNM3 is 3.4 in SMMC-7721,1.6 in HepG2 and 4.5 in McF-7(SMMC-7721 vs.HepG2,t=3.653,P<0.05;SMMC-7721 vs.McF-7,t=0.915,P>0.05);20μg/L CCL18 can effectively promote invasion and migration of hepatoma cell line SMMC-7721 with PITPNM3 high expression,by suppressing the expression of E-Cadherin and up-regulating the expressions of Snail and Vimentin;Overexpression of Mfn-2 leads to a significant decrease in PITPNM3 expression and suppression on invasion and migration in hepatoma cell line SMMC-7721,but has no effects on the mRNA level of PITPNM3.The relative expression level of PITPNM3 is 0.903 in experimental group and 1.128 in positive control group(experimental group vs.positive control group,P>0.05).Conclusion Mfn-2 can effectively down-regulate the expression of PITPNM3 and inhibit the invasion and migration of hepatoma cell line SMMC-7721.

关 键 词：线粒体融合蛋白2 膜相关磷脂酰肌醇转移蛋白3 肝癌 侵袭 迁移 

分 类 号：R735.7[医药卫生—肿瘤]