Shexiang Tongxin Dropping Pill（麝香通心滴丸）Protects against Na2S2O4-Induced Hypoxia-Reoxygenation Injury in H9c2 Cells  

作　　者：LIN Shan LIN Jiu-mao ZHANG Ling CHEN Da-xin XIAO Fei CHEN Hong-wei CHEN You-qin ZHU Yu-ling CHU Jian-feng PENG Jun 

机构地区：[1]Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou (350122), China [2]Fujian Key Laboratory of Integrative Medicine on Geriatric, Fujian University of Traditional Chinese Medicine, Fuzhou (350122), China [3]Rainbow Babies Children's Hospital, Case Western Reserve University School of Medicine, Cleveland, OH (44106), USA [4]Inner Mongolia Conba Pharmaceutical Co., Ltd., Shanghai (201318), China [5]CHEN Ke-ji Academic Thought Heritage Studio, Fuzhou (350122), China

出　　处：《Chinese Journal of Integrative Medicine》2019年第6期439-445,共7页中国结合医学杂志（英文版）

基　　金：Supported by the Foundation of Fujian University of Traditional Chinese Medicine(No.X2013026);the Developmental Fund of Chen Ke-ji Integrative Medicine(No.CKJ2013016);the Education Department of Fujian Province(No.JA14163)

摘　　要：Objectives: To investigate the protective effects of Shexiang Tongxin Dropping Pill(麝香通心滴丸,STP) on Na2S2O4-induced hypoxia-reoxygenation injury in cardiomyoblast H9c2 cells. Methods: The cell viability and levels of mRNA and protein expression in H9c2 cells were determined following Na2S2O4-induced hypoxia using Hoechst staining, annexin V/propidium iodide(PI) flow cytometry, real-time polymerase chain reaction and Western blot analysis. Results: STP pretreatment signi?cantly increased the viability and inhibited aberrant morphological changes in H9c2 cardiomyoblast cells induced by Na2S2O4 treatment(P<0.05). In addition, STP pretreatment attenuated Na2S2O4-induced hypoxic damage, down-regulated the expression of pro-apoptotic Bax,and up-regulated the expression of anti-apoptotic Bcl-2 in H9c2 cells(P<0.05). Conclusions: STP was strongly cardioprotective in hypoxia-reoxygenation injury by preventing hypoxic damage and inhibiting cellular apoptosis.These results further support the use of STP as an effective drug for the treatment of ischemic heart disease.Objectives: To investigate the protective effects of Shexiang Tongxin Dropping Pill（麝香通心滴丸,STP） on Na2S2O4-induced hypoxia-reoxygenation injury in cardiomyoblast H9c2 cells. Methods: The cell viability and levels of mRNA and protein expression in H9c2 cells were determined following Na2S2O4-induced hypoxia using Hoechst staining, annexin V/propidium iodide（PI） flow cytometry, real-time polymerase chain reaction and Western blot analysis. Results: STP pretreatment signi?cantly increased the viability and inhibited aberrant morphological changes in H9c2 cardiomyoblast cells induced by Na2S2O4 treatment（P<0.05）. In addition, STP pretreatment attenuated Na2S2O4-induced hypoxic damage, down-regulated the expression of pro-apoptotic Bax,and up-regulated the expression of anti-apoptotic Bcl-2 in H9c2 cells（P<0.05）. Conclusions: STP was strongly cardioprotective in hypoxia-reoxygenation injury by preventing hypoxic damage and inhibiting cellular apoptosis.These results further support the use of STP as an effective drug for the treatment of ischemic heart disease.

关 键 词：myocardial ISCHEMIA apoptosis Shexiang Tongxin DROPPING PILL Chinese medicine Na2S2O4-induced HYPOXIA-REOXYGENATION injury 

分 类 号：R285[医药卫生—中药学]