F-VEP评估大鼠视神经损伤后不同时间窗高压氧治疗效果  

Effects of hyperbaric oxygen therapy at different time windows after optic nerve injury in rats by F-VEP

作　　者：龙冰 周丽琼[2] 晏聃 罗生平 查旭[2] 曹霞[3] 李璋 孙晓莹 李霁 张远平[2] Long Bing;Zhou Liqiong;Yan Dan;Luo Shengping;Zha Xu;Cao Xia;Li Zhang;Sun Xiaoying;Li Ji;Zhang Yuanping(Department of Hyperbaric Oxygen and Department of Ophthalmology of Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China)

机构地区：[1]昆明医科大学第二附属医院高压氧科,昆明 [2]昆明医科大学第二附属医院眼科,昆明 [3]昆明医科大学第二附属医院中心实验室,昆明

出　　处：《中华航海医学与高气压医学杂志》2019年第2期108-111,115,共5页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：云南省科技厅-昆明医科大学应用基础研究联合专项资金项目(2017FE467-185);云南省教育厅科学研究基金资助项目(2014C043Y).

摘　　要：目的观察成年大鼠外伤性视神经损伤(traumatic optic nerve injury, TON)后不同时间点介入高压氧(hyperbaric oxygen,HBO)治疗后的闪光视觉诱发电位(flash visual evoked potential,F-VEP),探寻最佳HBO治疗时间窗。方法 40只成年大鼠分为5组,每组8只,按照视神经夹持模型HBO治疗介入时间点(第1、3、7、14天)不同设置实验HBO 1、2、3、4组和对照组。分别在治疗过程中的第3、10、20天监测5组大鼠双眼的F-VEP变化并进行疗效评估。结果对照组中夹持视神经损伤眼与假手术眼测得F-VEP数据比较,潜伏期时值延长及振幅均明显降低,差异有统计学意义(P<0. 05);与对照组F-VEP数据比较,实验1组视神经夹持眼各时间点潜伏期明显缩短;治疗第10天及第20天振幅明显增高,差异均有统计学意义(P<0. 05),而实验2组视神经夹持眼第10、20天潜伏期明显缩短,差异有统计学意义(P<0. 05),但振幅比较差异无统计学意义(P>0. 05)。实验3、4组视神经夹持眼中F-VEP数据与对照组比较,潜伏期时值及振幅变化差异均无统计学意义(P>0. 05)。结论早期HBO治疗能有效改善TON模型大鼠F-VEP,且在视神经损伤后越早介入HBO治疗效果越明显。Objective To observe flash visual evoked potential (F-VEP)after hyperbaric oxygen (HBO) at different time points after traumatic optic nerve injury(TON) in adult rats, so as to explore the optimal time window for HBO therapy. Methods A total of 40 Sprague-Dawley rats were used to establish an optic nerve contusion model, with the left eye as the optic nerve injury eye and the right eye as the sham surgery control eye. In accordance with different HBO intervention time points of the optic nerve contusion model at 1, 3, 7 and 14 days, and the animals were divided into 5 groups, i. e. the experimental group 1, group 2, group 3, group 4 and the control group, each consisting of 8 rats. In the course of treatment, F-VEP visual changes in the 5 groups were detected at day 3, day 10 and day 20, and therapeutic effects were evaluated. Results When the detected VEP data of the optic nerve-clamped eye in all the control rats were compared with those of the sham surgical eyes, latent time was prolonged and vibration amplitude was obviously decreased, and statistical significance could be found, when comparisons were made between them (P<0.05). Compared with the F-VEP data of the control group, latent time of the optic nerve-clamped eye in the experimental group 1 at various time points was considerably shortened, and at day 10 and day 20 after treatment, vibration amplitude was significantly increased, all with statistical significance (P<0.05). However, latent time of the optic nerve-clamped eye in the experimental group 2 at day 10 and day 20 after treatment was obviously shortened, also with statistical significance (P<0.05), but no statistical significance could be noted in vibration amplitude, when comparisons were made between them (P>0.05). When compared with the F-VEP data of the control group, no statistical significance could be found in the changes of latent time and vibration amplitude for the experimental group 3 and group 4 (P>0.05). Conclusion Early HBO treatment could effectively improve F-VEP of the rats wi

关 键 词：视神经损伤 高压氧 不同时间点 视觉诱发电位 

分 类 号：R774.6[医药卫生—眼科]