干旱胁迫下青稞全基因组可变剪切分析  

Genome-wide Variable Shear Analysis of Highland Barley under Drought Stress

作　　者：曾兴权 原红军[1,2] 杨春葆 于明寨[1,2] 徐齐君 王玉林 扎桑 尼玛扎西[1,3] ZENG Xing-quan;YUAN Hong-jun;YANG Chun-bao;YU Ming-zhai;XU Qi-jun;WANG Yu-lin;Zhasang;Nimazhaxi(State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement,Tibet Lhasa 850002,China;Institute of Agricultural Research,Tibet Academy of Agricultural and Animal Husbandry Sciences,Tibet Lhasa 850002,China;Tibet Academy of Agricultural and Animal Husbandry Sciences,Tibet Lhasa 850002,China)

机构地区：[1]省部共建青稞和牦牛种质资源与遗传改良国家重点实验室,西藏拉萨850002 [2]西藏自治区农牧科学院农业研究所,西藏拉萨850002 [3]西藏自治区农牧科学院,西藏拉萨850002

出　　处：《西藏农业科技》2019年第1期1-5,共5页Tibet Journal of Agricultural Sciences

基　　金：西藏财政专项(XZNKY-2018-C-021)

摘　　要：为阐明青稞在旱胁迫下基因发生可变剪切的规律,本研究以抗旱的“喜拉16号”和旱敏感的“迪庆黑元桂”为试验材料,分别对对照和21%PEG浓度下处理的样本进行多时间点的全转录组测序。结果表明,利用LeafCutter软件在所有的样品中共检测到了22181个可变剪切事件;通过PCA分析发现,可变剪切具有明显的品种间特异性;另外,通过与抗旱基因数据库比对分析发现,在干旱胁迫处理下2个类型品种间有多个抗旱相关的基因发生了显著的差异可变剪切,分别是HVUL1H16429(AtrbohF)、HVUL1H12808(HAB1)、HVUL4H58649(ABF4)、HVUL4H35985(AtrbohD)、HVUL7H07799(LOS5)、HVUL3H43774(CLCc)、HVUL3H23631(ABCG40)、HVUL1H16840(MYB60)和HVUL6H08236(AREB1);对2个品种各自在对照与旱胁迫条件下的差异可变剪切基因进行pathway分析,发现了抗旱品种的差异可变剪切基因参与了更多的pathway,并且鉴别出Fatty acid degradation、Inositol phosphate metabolism、alpha-Linolenic acid metabolism和Fatty acid metabolism这4条通路显著富集。为解析青稞抗旱分子机理与培育青稞抗旱新品种奠定了理论基础。In order to study the pattern of variable shearing of genes in highland barley under drought stress, the drought-resistant‘Xila 16’ and drought-sensitive‘Diqing Heiyuangui’were used as experimental materials.The samples processed at control and 21 % PEG concentrations were separately sequenced at multiple time points.The results showed that a total of 22 181 variable shearing events were detected in all samples by Leaf Cutter software, and it was also found by PCA analysis that the variable shearing has obvious specificity among varieties.In addition, by comparing with drought resistance gene database, there were significant differences in drought resistance related genes between the two varieties under drought stress: HVUL1H16429( AtrbohF), HVUL1H12808( HAB1), HVUL4H58649( ABF4), HVUL4H35985 ( AtrbohD), HVUL7H07799( LOS5), HVUL3H43774 ( CLCc), HVUL3H23631 ( ABCG40), HVUL1H16840 ( MYB60) and HVUL6H08236 ( AREB1).Comparing the variable shearing genes between two varieties by KEGG analysis, the drought-resistant varieties were involved in more pathways, and four significant enrichment pathways were identified, which were fatty acid degradation, inositol phosphate metabolism, alpha-Linolenic acid metabolism and fatty acid metabolism pathways.The research lays a theoretical foundation for analyzing the molecular mechanism of drought resistance of highland barley and breeding new varieties.

关 键 词：青稞 干旱胁迫 全基因组 可变剪切分析 

分 类 号：S512.3[农业科学—作物学]