青光安对抗青光眼术后滤过道瘢痕化中胶原纤维、α-SMA及FN的影响  

Effect of Qingguang’an on collagen fiber, alpha-SMA and FN in scar of filter tract after glaucoma surgery

作　　者：黄学思[1,2] 彭俊[1,3] 蒋鹏飞 李苑碧[1,3] 喻娟 彭清华 Xue-Si Huang;Jun Peng;Peng-Fei Jiang;Yuan-Bi Li;Juan Yu;Qing-Hua Peng(Hunan University of Chinese Medicine,Changsha 410208,Hunan Province,China;Department of Ophthalmology,the First Affiliated Hospital of Hunan College of Chinese Medicine,Zhuzhou 412000,Hunan Province,China;Hunan Provincial Key Laboratory of Otorhinolaryngology and Laryngeal Diseases,Changsha 410208,Hunan Province,China)

机构地区：[1]湖南中医药大学,湖南省长沙410208 [2]湖南中医药高等专科学校附属第一医院眼科,湖南株洲412000 [3]中医药防治眼耳鼻咽喉疾病湖南省重点实验室,湖南长沙410208

出　　处：《国际眼科杂志》2019年第6期906-910,共5页International Eye Science

基　　金：基金项目:国家自然科学基金资助项目(No.81273807,81603665);教育部博士学科点基金(No.20124323110006);中国博士后基金资助项目(No.2017M612565);国家中医药管理局中医眼科学重点学科建设项目;湖南省自然科学基金重点资助项目(No.11JJ2050,2017JJ3238);湖南省研究生创新基金重点资助项目(No.CX2013A013);湖南省高层次卫生人才“225”工程培养项目资助;湖南省中医五官科学重点学科建设项目;湖南中医药大学中医学一级学科资助(No.2018ZYX15)~~

摘　　要：目的:观察青光安4种有效组份对抗兔眼青光眼术后滤过道胶原纤维、α-平滑肌肌动蛋白(α-SMA)和纤维连接蛋白(FN)的影响。方法:将青光安4种有效组份与青光安中药混悬液应用于滤过手术后D组(有效组份1组)、E组(有效组份2组)、F组(有效组份3组)、G组(有效组份4组)、H组(青光安混悬液组),通过与A组(空白对照组)、B组(模型组)和C组(丝裂霉素C组)进行比较,观察青光安4种有效组份与青光安中药混悬液对青光眼术后滤过道瘢痕组织中胶原纤维、α-SMA及FN的影响。结果:C组、E组、F组、H组胶原纤维面积比值、α-SMA的表达、FN的表达与B组比较均有差异(P<0.05)。结论:青光安有效组份2、青光安有效组份3、丝裂霉素C以及青光安混悬液通过抑制胶原纤维、α-SMA及FN的表达,表现出明显的抗青光眼术后滤过道瘢痕化的作用。AIM: To observe the effects on four effective components of Qingguang an on collagen fibers,α-smooth muscle actin (α-SMA) and fibronectin (FN) in rabbits after glaucoma surgery. METHODS: Apply four kinds of effective components of Qingguang an and Qingguang an Chinese medicine suspension to D, E, F, G, H groups after filtration surgery, and pass with group A (blank control group) and group B (model group) Compared with group C (Mitomycin C group), the effects of four effective components of Qingguang an and Qingguang an traditional Chinese medicine suspension on collagen fibers,α-SMA and FN in the scar tissue of glaucoma after filtration were observed. RESULTS: Compare to B group, the ratio of collagen fiber area to E, F, H group, the expressions of α-SMA and the expressions of FN were different( P <0.05). CONCLUSION: Qingguang an effective components 2, Qingguang an effective components 3, mitomycin C and Qingguang an suspension reduce the proliferation of myofibroblasts and fibroblasts by inhibiting the expression of collagen fibers,α-SMA and FN, and showed obvious anti-glaucoma staining scar after surgery.

关 键 词：青光眼 滤过道瘢痕化 胶原纤维 Α-平滑肌肌动蛋白 纤维连接蛋白 

分 类 号：R779.6[医药卫生—眼科]