miR-194通过调控相关靶向蛋白影响肝癌细胞的增殖和侵袭行为  

miR-194 Regulates the Proliferation and Invasion of Hepatocellular Carcinoma Cells by Regulating Related Target Proteins

作　　者：张彬[1] 张瑜 ZHANG Bin;ZHANG Yu(Department of Hepatobiliary Surgery, Chengdu Fifth People’s Hospital, Sichuan Province 611130)

机构地区：[1]四川省成都市第五人民医院肝胆外科

出　　处：《医学理论与实践》2019年第10期1453-1455,1464,共4页The Journal of Medical Theory and Practice

摘　　要：目的:探讨miR-194通过调控相关靶向蛋白影响肝癌细胞的增殖和侵袭行为及其机制。方法: qPCR检测miR-194在肝癌组织和不同肝癌细胞株中的表达情况;Western blotting检测miR-194对钙粘蛋白表达水平的影响;划痕愈合实验和Transwell侵袭实验检测miR-194的表达对肝癌细胞的迁移和侵袭能力的影响;抑制miR-194的表达可以干扰肝癌细胞的生长发展进程。结果:与癌旁正常组织相比,miR-194在肝癌组织中的表达上调,miR-194在HepG2细胞株中表达水平相对较高;miR-194可直接调控相关钙粘蛋白的表达情况,抑制miR-194的表达后可以一定程度上抑制肝癌细胞的迁移和侵袭能力。结论: miR-194可以调控靶向蛋白的表达,影响肝癌细胞的迁移和侵袭行为。Objective: To investigate the effect and mechanism of miR-194 on the proliferation and invasion of hepatocellular carcinoma cells by regulating related target proteins. Methods: qPCR was used to detect the expression of miR-194 in hepatocellular carcinoma and different hepatocellular carcinoma cell lines. Western blotting detection of miR-194 on cadherin expression levels;Scratch healing assay and Transwell invasion assay to detect the expression of miR-194 on the migration and invasion of hepatocellular carcinoma cells;Inhibition of the expression of miR-194 can interfere with the growth and development of hepatocellular carcinoma cells. Results: The expression of miR-194 was up-regulated in HCC tissues compared with the adjacent normal tissues.The expression level of miR-194 in HepG2 cells was relatively high. miR-194 can directly regulate the expression of related cadherin, Inhibition of the expression of miR-194 can inhibit the migration and invasion of hepatocellular carcinoma cells to a certain extent. Conclusion: miR-194 can regulate the expression of target proteins and effect the migration and invasion behavior of liver cancer cells.

关 键 词：miR-194 肝癌 增殖行为 迁移能力 

分 类 号：R735.7[医药卫生—肿瘤]