基于Miseq测序的长江中游枯水季水体菌群结构  

The Water Microbial Community Structure in Dry Season of the Middle Reaches of Yangtze River Based on Miseq Sequencing

作　　者：梁杰超 季斌[1] 陈威[1] 宋宏娇[1] 姚嘉雄 龚喜平 肖曹帆 刘扬 LIANG Jie-chao;JI Bin;CHEN Wei;SONG Hong-jiao;YAO Jia-xiong;GONG Xi-ping;XIAO Cao-fan;LIU Yang(School of Urban Construction, Wuhan University of Science and Technology, Wuhan 430065, China)

机构地区：[1]武汉科技大学城市建设学院

出　　处：《科学技术与工程》2019年第14期84-89,共6页Science Technology and Engineering

基　　金：长江水利委员会长江科学院开放研究基金(CKWV2018490/KY);湖北省自然科学基金(2017CFB350)资助

摘　　要：采用基于16S rRNA基因的Illumina Miseq测序方法,对长江中游枯水季冬季的水质及水体细菌群落多样性进行系统分析。研究发现,所测长江水段当季属于《地表水环境质量标准》(GB 3838-2002)Ⅲ类水。长江中菌群多样性高,变形菌门(Proteobacteria,44. 9%)、β-变形菌纲(Betaproteobacteria,32. 9%)以及丛毛单胞菌科(Comamonadaceae,30. 6%)分别在门、纲、科上为优势菌。除此之外,长江水体中大量的优势菌科与生物除氮相关,其中具有好氧反硝化功能的丛毛单胞菌科(Comamonadaceae)相对丰度最大,对于控制水体中氮素的污染指标,促进水体自净修复有积极的作用。PICRUSt功能预测分析表明水体中菌群在碳水化合物代谢和氨基酸代谢方面功能最强。在菌种关联网络中,拟杆菌属(Bacteroides)、黄杆菌属(Luteolibacter)、Pseudarcicella等在共存网络菌群中具有正相关的关系,可以抑制微囊藻的生长。研究揭示了长江枯水季的水质与其微生物群落结构的内在关系,可以为长江枯水季饮用水源地水质安全保障提供理论依据。The water quality and the diversity of bacteria community in the dry season and winter of the Yangtze river were systematically analyzed by using the Illumina Miseq sequencing method based on 16 S rRNA gene. The show that the test of the Yangtze River water belongs to the Ⅲ GB 3838-2002 class. The diversity of the bacteria community in the Yangtze River is high,with Proteobacteria( 44. 9%),protein-proteobacteria( 32. 9%) and Comamonadaceae( 30. 6%) as dominant species in the phylum,class and family. In addition,a large number of dominant bacteria families in the water of the Yangtze River are related to biological nitrogen removal. Among them,Comamonadaceae with aerobic denitrification has the largest relative abundance,which plays a positive role in controlling the pollution index of nitrogen in water and promoting the self-purification and repair of water.PICRUSt function prediction showed that bacteria in water had the strongest function in carbohydrate and amino acid metabolism. Bacteroides,Luteolibacter and Pseudarcicella are positively correlated in the network of bacterial species association,which can inhibit the growth of microcystis. The results reveal the internal relationship between the water quality of the Yangtze River dry season and the microbial community structure,which can provide theoretical basis for the water quality security of the drinking water sources of the Yangtze River dry season.

关 键 词：长江 枯水季 水质 微生物多样性 丛毛单胞菌 黄杆菌属 

分 类 号：Q938.8[生物学—微生物学]