不同剂量三参饮改善干燥及NOD小鼠颌下腺PD-1及PD-L1高表达情况  

Clinical effective of different doses of San Shen Yin treatment on Sjogren’s syndrome and improvement of expression of PD-1 and PD-L1 on animal models’ salivary glands

作　　者：陈月月 汪悦 钱冬晨 何静 郭亮 徐媚媚 胡伟 徐蕾 CHEN Yue-Yue;WANG Yue;QIAN Dong-Chen;HE Jing;GUO Liang;XU Mei-Mei;HU Wei;XU Lei(The Third Affiliated Hospital of Nanjing University of Traditional Chinese Medicine,Nanjing 210001,China)

机构地区：[1]南京中医药大学第三附属医院

出　　处：《中国免疫学杂志》2019年第10期1193-1198,共6页Chinese Journal of Immunology

基　　金：2017南京市计委课题(YKK17152)

摘　　要：目的:研究不同剂量三参饮治疗干燥综合征患者疗效,并在干燥综合征动物模型上研究不同剂量三参饮治疗后,其颌下腺PD-1及PD-L1高表达情况。方法:自2013年4月至2018年4月,在我院就诊原发性干燥综合征患者共110例,根据治疗剂量随机分为高剂量组、中剂量组和低剂量组,予以不同剂量的三参饮治疗,观察疗效及不良反应情况。而后,利用不同剂量三参饮喂养干燥综合征模型鼠,免疫组化观察其颌下腺中PD-1及PD-L1表达情况。结果:予以三参饮辅助干燥综合征治疗,可明显改善患者口咽、眼部、鼻腔等部位的干燥症状。同时高剂量组疗效优于低剂量组,组间比较差异有统计学意义。在干燥综合征模型鼠中,予以三参饮灌胃,可降低小鼠颌下腺中PD-1及PD-L1表达,同时高剂量组PD-1及PD-L1表达较中、低剂量组降低明显(P<0.05)。结论:三参饮治疗可明显改善干燥综合征患者临床症状,高剂量治疗效果更佳,此外,三参饮可能通过对免疫细胞调节发挥临床作用。Objective: To study the clinical effective of different doses of San Shen Yin treatment on the Sjogren′s syndrome and the improvement of the expression of PD-1 and PD-L1 on salivary glands. Methods: From April,2013 to April,2018,110 Sjogren′s Syndrome patients were recruited into the study.They were divided into three groups:High Dose Group,Middle Dose Group and Low Dose Group randomly and accepted different doses of San Shen Yin treatment.Then,patients were followed up and monitor their clinical effective.At the same time,we set up mice model of Sjogren′s Syndrome.These mice were feed by high,middle and low doses San Shen Yin.After that,the expressions of PD-1 and PD-L1 in salivary glands of mice were observed by immunohistochemistry. Results: The San Shen Yin treatment could improve patients′ clinical symptoms of dryness in the oropharynx,eyes,nasal cavity.Compared with the Low Dose Group,patients in the High Dose Group have got better improvement in the clinical effective.The significant difference was found in the different groups patients.In Sjogren′s Syndrome mice,the San Shen Yin treatment reduced the expressions of PD-1 and PD-L1 in salivary glands.The High Dose Group mice had the lowest expressions of PD-1 and PD-L1 ( P <0.05). Conclusion: The San Shen Yin improved the clinical effective in Sjogren′s Syndrome patients,and the higher dose of the San Shen Yin got better improvement of the clinical symptoms.The San Shen Yin might play its role via the modification of the immune system.

关 键 词：三参饮 干燥综合征 PD-1 PD-L1 

分 类 号：R255R364[医药卫生—中医内科学]