长链非编码RNA MAPKAPK5-AS1对肺癌细胞的侵袭和凋亡的影响  

Effect of long non-coding RNA (lncRNA) MAPKAPK5-AS1 on cellular invasion and apoptosis in lung cancer cells

作　　者：孙一奎[1] 赵枫[1] 吕绮 王卫华[1] SUN Yi-kui;ZHAO Feng;LV Qi;WANG Wei-hua(The Affiliated Hospital of Medical School of Ningbo University,Ningbo,Zhejiang 315000,China)

机构地区：[1]宁波大学医学院附属医院

出　　处：《中国卫生检验杂志》2019年第9期1025-1028,共4页Chinese Journal of Health Laboratory Technology

基　　金：浙江省宁波市自然科学基金项目(2015A610237);浙江省自然科学基金一般项目(Y17H160042)

摘　　要：目的研究长链非编码RNA(long ncRNA,lncRNA)MAPKAPK5-AS1对肺癌细胞的侵袭和凋亡的影响。方法real-time PCR法筛选MAPKAPK5-AS1高表达和低表达肺癌细胞系,将MAPKAPK5-AS1过表达载体转染低表达细胞系,MAPKAPK5-AS1小干扰RNA(small interference RNA,siRNA)转染高表达细胞系,Western blot分别检测宿主基因MAPKAPK5和凋亡蛋白表达,transwell实验分析细胞侵袭能力。结果MAPKAPK5-AS1在肺癌H2291细胞株和A549细胞株中高表达,而在H441细胞株中低表达。在H441细胞中过表达MAPKAPK5-AS1后,MAPKAPK5表达明显下降,侵袭能力明显增强(F=319.5,P<0.01),凋亡蛋白Cleavedcaspase 9的表达下降;而在H2291细胞和A549细胞中分别干扰MAPKAPK5-AS1表达后,MAPKAPK5表达明显升高,侵袭能力明显下降(F值分别为417.1、530.2,P<0.01),凋亡蛋白Cleaved caspase 9的表达升高。结论MAPKAPK5-AS1可能作为原癌基因通过调控宿主基因MAPKAPK5表达而增强肺癌细胞侵袭能力,同时抑制细胞凋亡。Objective To investigate the effects of long-chain non-coding RNA ( long ncRNA,lncRNA) MAPKAPK5-AS1 on invasion and apoptosis of lung cancer cells. Methods Real time PCR was performed to screen the lung cancer cells with high or low expression MAPKAPK5-AS1,MAPKAPK5-AS1 over expression vector or MAPKAPK5-AS1 small interference RNA ( siRNA) was transfected into the screened lung cancer cells,respectively. The host gene MAPKAPK5 and apoptosis proteins were detected by western blot,the cellular invasive ability was analyzed by transwell assay. Results MAPKAPK5-AS1 expression was relatively higher in the H2291 and A549 cells than the H441 cells. After MAPKAPK5-AS1 over expression in the H441 cells,host gene MAPKAPK5 expression was inhibited,the cellular invasive ability was increased ( F = 319. 5, P < 0. 01),and apoptosis protein cleaved caspase 9 expression was decreased. Conversely,after MAPKAPK5-AS1 siRNA tansfection in the H2291 and A549 cells,host gene MAPKAPK5 expression was increased,the cellular invasive ability was weakened ( F = 417. 1,F = 530. 2,P < 0. 01),and apoptosis protein cleaved caspase 9 expression was increased. Conclusion MAPKAPK5-AS1 may act as an oncogene to promote cellular invasion and inhibit cellular apoptosis of lung cancer cells by down regulating host gene MAPKAPK5 expression.

关 键 词：长链非编码RNA MAPKAPK5-AS1 肺癌 侵袭 凋亡 

分 类 号：R734.2[医药卫生—肿瘤]