珍贵束丝放线菌产生的新代谢产物1-羟基-4-O-鼠李糖-2-萘甲酸甲酯  

A novel metabolite methyl 1-hydroxy-4-O-rhamnosyl-2-naphthoate from Actinosynnema pretiosum

作　　者：王静[1] 胡笑文 白利平[1] 张苗青 张靖溥[1] 江冰娅[1] 孙桂芝[1] 黎林丽 李书芬[1] 张玉琴[1] 刘红宇[1] 余利岩[1] 武临专[1] WANG Jing;HU Xiao-wen;BAI Li-ping;ZHANG Miao-qing;ZHANG Jing-pu;JIANG Bing-ya;SUN Gui-zhi;LI Lin-li;LI Shu-fen;ZHANG Yu-qin;LIU Hong-yu;YU Li-yan;WU Lin-zhuan(NHC Key Laboratory of Biotechnology of Antibiotics,CAMS Key Laboratory of Synthetic Biology for Drug Innovation,Institute of Medicinal Biotechnology,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100050,China)

机构地区：[1]中国医学科学院北京协和医学院医药生物技术研究所卫健委抗生素生物工程重点实验室/中国医学科学院药物合成生物学重点实验室

出　　处：《中国医药生物技术》2019年第3期210-214,共5页Chinese Medicinal Biotechnology

基　　金：“重大新药创制”国家科技重大专项(2018ZX09711001);国家微生物资源平台(NIMR-2018-3)

摘　　要：目的发现珍贵束丝放线菌ATCC 31565产生的新代谢产物。方法TLC和LC-MS等分析珍贵束丝放线菌ATCC 31565培养物的乙酸乙酯提取物,发现目标化合物。采用MCI色谱柱和半制备HPLC等分离纯化目标化合物。对目标化合物的NMR等数据进行分析,确定其化学结构。结果从珍贵束丝放线菌ATCC 31565中发现了一个化学结构为1-羟基-4-O-鼠李糖-2-萘甲酸甲酯的新化合物,并将其命名为珍贵酚。结论推测珍贵酚是1,4-二羟基-2-萘甲酸(甲萘醌的生物合成前体物)和L-鼠李糖这两个初级代谢产物的脱水缩合产物再经过甲酯化形成的。Objective To discover novel metabolite(s)from Actinosynnema pretiosum ATCC 31565.Methods TLC and LC-MS were used to analyze the EtOAc extract of fermentation culture of ATCC 31565.MCI column chromatography and HPLC were employed for the isolation of novel metabolite.The structure of the metabolite was elucidated by NMR.Results A new metabolite,designated as pretiphenol,was discovered from Actinosynnema pretiosum ATCC 31565.The structure of pretiphenol was determined as methyl 1-hydroxy-4-O-rhamnosyl-2-naphthoate.Conclusion Pretiphenol might be the dehydration product of 1,4-dihydroxy-2-naphthoate(an intermediate in menaquinone biosynthesis)and L-rhamnose,followed by esterification with methanol.

关 键 词：珍贵束丝放线菌ATCC 31565 珍贵酚 1 4-二羟基-2-萘甲酸 鼠李糖 

分 类 号：R915[医药卫生—微生物与生化药学]