活体视网膜神经节细胞标记和显像方法  

In vivo labeling and imaging of retinal ganglion cell

作　　者：邢小丽 黄亮瑜 东莉洁 李筱荣 Xing Xiaoli;Huang Liangyu;Dong Lijie;Li Xiaorong(Tianjin Medical University Eye Hospital,Tianjin 300384,China)

机构地区：[1]天津医科大学眼科医院,300384

出　　处：《国际眼科纵览》2019年第2期73-78,共6页International Review of Ophthalmology

基　　金：天津医科大学眼科研究所科研临床基金(15YKYJS001);天津医科大学校级基金(2006KY27).

摘　　要：视网膜神经节细胞(retina ganglion cells,RGC)损伤是多种视网膜疾病发展的必然过程。RGC的形态和数量通常是相关研究的重要指标。目前RGC活体标记方法主要有逆行标记、玻璃体注射荧光染料标记凋亡RGC细胞、转基因小鼠、电穿孔、共焦激光扫描眼底镜(confocal scanning laserophthalmoscopy,CSLO)、相干光断层扫描(optical coherence tomography,OCT)、基因编码钙离子指示剂(genetically encoded indicators,GECI)成像以及功能性自适应光学活体细胞成像等,有些标记方法已用于临床研究。Retinal ganglion cells (RGC) damage is an important part of the development of many other retinal and optic nerve diseases.Research on RGC is always a hot topic.RGC labeling methods in vivo mainly include retrograde labeling,intravitreal injection of fluorescence dyes,transgenic mice,electroporation,confocal scanning laser ophthalmoscope (CSLO) combined with fluorescent labeling,optical coherence tomography (OCT),genetically encoded indicators (GECI) imaging,and functional adaptive optical living cell imaging etc.Some labling methods are used in clinical trails.

关 键 词：视网膜神经节细胞 标记 

分 类 号：R774.1[医药卫生—眼科] R775[医药卫生—临床医学]