HPLC法同时测定枸骨叶中10种多酚类成分的含量  

Simultaneous Determination of 10 Polyphenols in the Leaves of Ilex cornuta by HPLC

作　　者：陈颖 王存琴 汪雷 臧莉 凌子萍 陈健 CHEN Ying;WANG Cunqin;WANG Lei;ZANG Li;LING Ziping;CHEN Jian(College of Pharmacy,Wannan Medical College,Wuhu 241002 Anhui,China)

机构地区：[1]皖南医学院药学院

出　　处：《中药新药与临床药理》2019年第6期715-718,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金项目(81402818);安徽省高校优秀青年人才支持计划重点项目(gxyqZD2016177);安徽省高等学校省级自然科学研究重点项目(2014A268)

摘　　要：目的建立HPLC同时测定枸骨叶中新绿原酸、绿原酸、隐绿原酸、咖啡酸、对羟基肉桂酸、芦丁、异槲皮苷、异绿原酸B、异绿原酸A和异绿原酸C10种多酚类成分含量的方法。方法采用YMC-ODSC18(4.6mm×250mm,5μm)色谱柱,乙腈-0.2%磷酸水溶液梯度洗脱,检测波长为320nm;柱温:40℃;流速:1.0mL·min^-1;进样量:10μL。结果10种多酚类成分的分离效果良好,质量浓度分别在8.19~163.80、3.20~64.00、2.76~55.20、2.31~46.20、4.33~86.60、4.91~98.20、3.03~60.60、2.88~57.60、2.48~49.60、5.09~101.80μg·mL^-1范围内呈良好的线性关系(r>0.9996),平均加样回收率(n=6)在97.37%~105.58%之间,RSD≤3.72%。结论该方法快速、准确、重复性好,可用于同时测定枸骨叶中10种多酚类成分的含量,为枸骨叶药材的质量控制提供科学依据。Objective To establish an HPLC method for simultaneous determination of isochlorogenic acid B,isochlorogenic acid A, isochlorogenic acid C, cryptochlorogenic acid, caffeic acid, p-hydroxycinnamic acid,rutin,isoquercitrin,neochlorogenic acid,and chlorogenic acid,the 10 different polyphenols in the leaves of Ilex cornuta. Methods YMC-ODS C18 column(4.6 mm×250 mm,5 μm)was used with gradient elution of acetonitrile-0.2% phosphoric acid water,a detection wavelength of 320 nm;column temperature of 40 °C,a flow rate of 1.0 mL·min-1,and injection volume of 10 μL. Results The separation of 10 polyphenols showed good linearities in the mass concentration ranges of 8.19-163.80, 3.20-64.00, 2.76-55.20, 2.31-46.20, 4.33-86.60, 4.91-98.20,3.03-60.60,2.88-57.60,2.48-49.60,5.09-101.80 μg·mL-1(r > 0.999 6). The average recoveries(n = 6)were97.37%-105.58,and RSD≤3.72. Conclusion The method is rapid,accurate and reproducible. It can be used for the simultaneous determination of 10 kinds of polyphenols in leaves of Ilex cornuta.

关 键 词：枸骨叶 多酚类化合物 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]