PUR PLISH法同时测定不同产地菟丝子中6种主要活性成分含量  

Simultaneous determination of six main active components in semen Cuscuta from different habitats by RP-HPLC

作　　者：张燕丽[1] 孟凡佳[1] 田园[1] 候建鹏 张晓娟[1] ZANG Yan-li;MENG Fan-jia;TIAN Yuan;HOU Jian-peng;ZHANG Xiao-juan(Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, China)

机构地区：[1]黑龙江中医药大学

出　　处：《化学工程师》2019年第5期29-33,共5页Chemical Engineer

基　　金：黑龙江省卫生计生委科研课题(No.2018583);黑龙江省自然科学基金项目(No.H201330)

摘　　要：建立了RP-HPLC法同时测定菟丝子中6种活性成分,金丝桃苷、异槲皮苷、紫云英苷、山奈酚、槲皮素和异鼠李素的含量。方法:采用高效液相色谱法,色谱柱为Diamonsil-C18(5μm, 4.6mm×250mm)色谱柱;以乙腈(A)-0.1%磷酸水(B)为流动相梯度洗脱(0~20min,15%A→28%A;20~30min,28%A→68%A;30~40min,68%A),流速为1.0mL·min^-1,进样量为10μL,柱温为30℃,检测波长为360nm。结果:6种成分在相应的线性范围内线性关系良好。精密度的RSD为0.73%~1.98%;加样回收率为95.37%~108.00%,RSD值为2.79%~3.31%;稳定性的RSD为0.64%~2.71%。结论:9批不同产地得菟丝子药材均含有所测的6种活性成分,但含量差异较大。该方法专属性强,稳定可靠,可用于菟丝子中6种活性成分的含量测定。Objective: A RP-HPLC method for simultaneous determination of six active components(hyperoside, isoquercitrin, astragalin, quercetin, kaempferol and isorhamnetin) in Cuscuta chinensis was established.Methods: High performance liquid chromatography method, This method use a Diamonsil-C18(5μm, 4.6 mm ×250 mm)as chromatographic column;mobile phase: acetonitrile(A)-0.1% phosphoric acid water(B), gradient elution(0~20 min, 15%A→28%A;20~30 min, 28%A→68%A;30~40 min, 68%A). The flow rate was set at 1.0 mL·min^-1;the sample size is 10μL;column temperature is 25℃;detection wavelength is 360 nm. Result: Six active components in the corresponding linear range were showing good linear relationship. The precision of RSD is 0.73%~1.98%;The sample recovery is 95.37%~108.00% and RSD is 2.79%~3.31%;The RSD of stability is 0.64%~2.71%. Conclusion: The 9 batches of Cuscuta chinensis from different habitats contain 6 active components, but the content is quite different. The method is specific, stable and reliable. It can be used to determine the content of 6 active components in Cuscuta chinensis.

关 键 词：菟丝子 含量测定 活性成分 皮肤病 药物化学 

分 类 号：R651.3[医药卫生—外科学]