The autophagy gene ATG8 affects morphogenesis and oxidative stress tolerance in Sporisorium scitamineum  

作　　者：ZHANG Bin CUI Guo-bing CHANG Chang-qing WANG Yi-xu ZHANG Hao-yang CHEN Bao-shan DENG Yi-zhen JIANG Zi-de 

机构地区：[1]Department of Plant Pathology/State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University [2]Integrative Microbiology Research Centre/Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University [3]College of Life Science and Technology/State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University

出　　处：《Journal of Integrative Agriculture》2019年第5期1024-1034,共11页农业科学学报（英文版）

基　　金：supported by the National 973 Program of China(2015CB150600);the Natural Science Foundation of Guangdong Province,China(2017A030310144)

摘　　要：The basidiomycetous fungus Sporisorium scitamineum causes sugarcane smut that leads to severe economic losses in the major sugarcane growing areas in China,India and Brazil,etc.Autophagy is a conserved pathway in eukaryotes for bulk degradation and cellular recycling,and was shown to be important for fungal cell growth,development,and pathogenicity.However,physiological function of autophagy has not been studied in S.scitamineum.In this study,we identified a conserved Atg8 protein,named as SsAtg8 and characterized its function.Our results showed that autophagy was blocked in the ssatg8Δ mutant,in nitrogen starvation.The ssatg8Δ mutant formed pseudohypha frequently and was hypersensitive to oxidative stress.However,mating or filamenation was unaffected in the ssatg8Δ mutant in vitro.Overall we demonstrate that autophagy is dispensable for S.scitamineum mating/filamentation,while critical for oxidative stress tolerance and proper morphology in sporidial stage.The basidiomycetous fungus Sporisorium scitamineum causes sugarcane smut that leads to severe economic losses in the major sugarcane growing areas in China,India and Brazil,etc.Autophagy is a conserved pathway in eukaryotes for bulk degradation and cellular recycling,and was shown to be important for fungal cell growth,development,and pathogenicity.However,physiological function of autophagy has not been studied in S.scitamineum.In this study,we identified a conserved Atg8 protein,named as SsAtg8 and characterized its function.Our results showed that autophagy was blocked in the ssatg8Δ mutant,in nitrogen starvation.The ssatg8Δ mutant formed pseudohypha frequently and was hypersensitive to oxidative stress.However,mating or filamenation was unaffected in the ssatg8Δ mutant in vitro.Overall we demonstrate that autophagy is dispensable for S.scitamineum mating/filamentation,while critical for oxidative stress tolerance and proper morphology in sporidial stage.

关 键 词：Atg8 AUTOPHAGY FUNGUS Sporisorium scitamineum MORPHOGENESIS OXIDATIVE stress TOLERANCE 

分 类 号：S[农业科学]