不同品种啤酒中黄腐酚含量差异性成因的研究  

The reasons for the difference of xanthohumol content in different kinds of beer

作　　者：赵惠新[1,2] 周茜 曾卫军[1] 卢函 赵和平[1,2] ZHAO Hui-xin;ZHOU Qian;ZENG Wei-jun;LU Han;ZHAO He-ping(Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology,Key Laboratory of Plant Stress Biology in Arid Land,College of Life Science,Xinjiang Normal University,Urumqi 830054;Beijing Key Laboratory of Gene Resource and Molecular Development,College of Life Science,Beijing Normal University,Beijing 100875;College of Life and Geography,Kashgar University,Kashi 844006,China)

机构地区：[1]新疆师范大学新疆特殊环境物种保护与调控生物学实验室干旱区植物逆境生物学实验室,乌鲁木齐830054 [2]北京师范大学抗性基因资源与分子发育北京市重点实验室,北京100875 [3]喀什大学生命与地理科学学院,喀什844006

出　　处：《生物学杂志》2019年第3期70-73,82,共5页Journal of Biology

基　　金：国家自然科学基金项目(No.31460041,No.U1703112);新疆师范大学“十三五”校级重点学科生物学学科开放课题(17SDKD0201);新疆师范大学研究生科研创新项目(XSY201702010)

摘　　要：为了探究不同品种啤酒中黄腐酚(Xanthohumol,Xn)含量不同的主要成因,设计了不同的遮光处理实验,对比了用6种不同啤酒及类似色度稀释黑墨水遮光处理对Xn样品的光异构化的影响。结果表明,用啤酒覆盖遮光,或混合啤酒能抑制Xn样品的光异构化,且黑啤的抑制效果优于浅色啤酒;稀释黑墨水覆盖遮光,也能抑制Xn样品的光异构化,效果与色度相近的黑啤一致。当添加了不同品种啤酒的Xn样品,用相同覆盖厚度的黑墨水进行遮光后,光照处理后,均能抑制光吸收值OD370降低,但各样品间并无显著性差异。研究间接证明了不同啤酒Xn含量差异的真正原因,在于透光性不同的物理作用,而不是含有小分子抑制剂的化学作用,这为优化啤酒发酵及储存的光照条件提高啤酒黄腐酚含量提供了借鉴。The aim of this study is to explore the main causes of different xanthohumol contents in different breeds. Six different beer and similar color dilution black ink shading experiments were conducted to compare their effects on the photoisomerization of xanthohumol samples. The results showed that any kind of beer covered or mixed into the sample could inhibit the photoisomerization of xanthohumol, and the inhibition effect of dark beer was better than that of light beer. In addition, the use of diluted black ink can also inhibit the photoisomerization of xanthohumol samples. The effect is consistent with the black beer with similar chromaticity. When adding different kinds of beer to xanthohumol samples and covering them with the same thickness of black ink, all varieties of beer could inhibit the decreasing of OD 370 , but there was no significant difference between all kinds of beer. And it implied that the real reason for the difference of xanthohumol content in different beer is that different brewers have different light transmittance, rather than the small molecule inhibitors in beer.

关 键 词：啤酒 黄腐酚 含量差异 光异构化 

分 类 号：Q819[生物学—生物工程]