栽培甘草的化学成分及其抗菌活性研究  

Study on Compounds and Antibacterial Activity of Cultivated Glycyrrhiza uralensis Fisch.

作　　者：包芳 白海英 彭静文 魏冠华 王丽瑶 邓庭伟 王继永 刘映前[1] 杨志刚 BAO Fang;BAI Hai-ying;PENG Jing-wen;WEI Guan-hua;WANG Li-yao;DENG Ting-wei;WANG Ji-yong;LIU Ying-qian;YANG Zhi-gang(School of Pharmacy Lanzhou University,Lanzhou 730030,China;China National of Traditional & Herbal Medicine Co.,Ltd.,Beijing 102600,China)

机构地区：[1]兰州大学药学院,甘肃兰州730030 [2]中国中药有限公司,北京102600

出　　处：《中国现代中药》2019年第5期577-582,共6页Modern Chinese Medicine

基　　金：国家重点研发计划(2016YFE0129000,2017YFC1701400,SQ2017YFC170424);国家中药标准化项目(ZYBZH-Y-ZY-45);国家中药材产业技术体系遗传改良研究室建设(CARS-21);中医药公益性行业科研专项(201507002-1-10);甘肃省重点研发计划(18YF1WA115);甘肃省中医药管理局科研课题(GZK-2015-21);兰州大学中央高校基本科研业务费项目(lzujbky-2017-k26)

摘　　要：目的:为进一步利用甘草资源,为开发安全、绿色和无污染的植物源杀菌剂提供依据,对栽培甘草的化学成分进行研究。方法:运用硅胶、葡聚糖凝胶LH-20(Sephadex LH-20)及制备型高效液相色谱仪(HPLC)等多种色谱方法对甘草成分进行分离纯化,根据其理化性质和波谱数据对化合物的结构进行鉴定,采用菌丝生长速率法对甘草素(10)、半甘草异黄酮B(11)、isoangustone A(12)和粗毛甘草素C(13)4个酚类化合物进行了4种常见农业植物病原菌(油菜菌核菌、立枯丝核菌、番茄灰霉菌和小麦赤霉菌)的抗菌活性筛选。结果:从甘草中分离鉴定出14个化合物,半甘草异黄酮B和粗毛甘草素C对农业植物病原菌的抑制率较高。结论:本次实验报道了化合物10~13对常见农业植物病原菌的抗菌活性,其中半甘草异黄酮B抗菌效果显著。Objective:In order to further utilize licorice resources,provide a basis for the development of safe,green and pollution-free plant source fungicides,chemical constituents of cultivated licorice from MinqinSome were studies.Methods:The constituents were isolated by means of silica gel,Sephadex LH-20 open column,and semipreparative HPLC.The structures were identified by their physical and chemical properties and spectroscopic data.Four common agricultural plant pathogens(Sclerotinia sclerotiorum,Rhizoctonia solani,Botrytis cinerea and Fusarium graminearum)were carried out for antibacterial activity screening of 4 phenolic compounds,liquiritigenin(10),semilicoisoflavone B(11),isoangustone A(12)and glyasperin C(13)by using the mycelial growth rate method.Results:14 compounds were isolated and identified from licorice.Semilicoisoflavone B and glyasperin C showed higher antibacterial rate.Conclusion:In this study,the antibacterial activities of compounds 10-13 against common agricultural plant pathogens were reported for the first time,and the antibacterial effect of semilicoisoflavone B was significant.

关 键 词：甘草 酚类化合物 半甘草异黄酮B 粗毛甘草素C 抗菌活性 

分 类 号：R284.1[医药卫生—中药学]