超高效液相色谱法区分四色喷墨打印墨迹  

Discrimination of Four-color Dye-based Inkjet Marks by UPLC

作　　者：沈畅 邹积鑫[2] 廉哲[2] 李世峰[2] 李杏茹[1] SHEN Chang;ZOU Jixin;LIAN Zhe;LI Shifeng;LI Xingru(Capital Normal University,Beijing 100048,China;Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China)

机构地区：[1]首都师范大学,北京100048 [2]公安部物证鉴定中心,北京100038

出　　处：《刑事技术》2019年第3期238-241,共4页Forensic Science and Technology

基　　金：“十三五”国家重点研发计划子课题(No.2016YFC0800705、No.2016YFC0800705-2)

摘　　要：目的建立不同品牌黄、品、青、黑四色喷墨打印墨迹的检验方法。方法采用BEH ShieldRP18色谱柱(2.1mm×100mm,1.7μm),流动相为乙腈-10mmol/L碳酸氢铵,梯度洗脱,流速为0.4mL/min,检测波长为400、540、585、600nm,采用超高效液相色谱法检验黄、品、青、黑四色喷墨打印墨迹。结果首先对126种黄品青黑四色喷墨打印墨迹进行溶解性实验,筛选出102种可溶性四色喷墨样品进行色谱实验,可将102种染料型彩色墨迹依据特征波长下主峰的保留时间将其分为46大类。不仅能将Canon、Epson、HP等三大品牌与非原装墨盒进行区分,不同品牌黄、品、青、黑四色也能分别得到较好的区分,区分率分别为72.0%、96.2%、52.0%、51.9%。某些同一品牌的样品也能得到区分。结论经过实际案件的检验,所建立的方法易于操作且灵敏度高,能用于彩色喷墨打印墨迹的检验。Objective To establish a UPLC method for distingu ishing the printed marks from 4-color (yellow,magenta, cyan and black) dye-based inkjet cartridges.Methods Four-color dyes of 126-kind inkjet cartridges from different manufacturers of their own trademarks were separated on a BEH Shield RP18 column (2.1mm×100mm,1.7μm) inset into a Waters Acquity UPLC apparatus.Gradient elution was carried out with the solution of acetonitrile and ammonium bicarbonate solution (NH4HCO3,10mmol/L) after the optimization of both extraction solvent and mobile phase.With the detection wavelengths set at 400nm,540nm,585 nm and 600 nm,the printed paper was investigated of its impact together with the printing ways on the four colors’ detection.In addition,the established method was examined of its precision and repeatability. Results Based on the differences among the dye components,102-kind dye-based coloring inkjet entries were categorized into 46 main classes.Conclusion The method is capable of providing an effective,reliable and stable choice for discrimination of different dye-based coloring inkjet entries.

关 键 词：染料型 黄、品、青、黑四色墨迹 保留时间 超高效液相色谱法 

分 类 号：DF794.2[政治法律—诉讼法学]